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Regenerative Applications of Stem Cells, PRP, Micrografts, and Biomaterials

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A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: closed (1 April 2020) | Viewed by 14135

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Special Issue Editor

Dr. Pietro Gentile

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Guest Editor

Associate Professor of Plastic and Reconstructive Surgery, Surgical Science Department, University of Rome “Tor Vergata”, Rome, Italy
Interests: regenerative plastic surgery; adipose-derived mesenchymal stem cells; fat grafting; platelet-rich plasma; growth factors; mesenchymal stem cells; biomaterials; hair loss; androgenetic alopecia
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Tissue engineering and regenerative plastic surgery are interrelated terms that often go hand-in-hand when discussing stem cells (mesenchymal and follicular), biotechnology and bioactive molecules (platelet-rich plasma (PRP), micrografts), and biomaterials (scaffolds, hydrogels) for hair re-growth, fat graft improvement, and/or regeneration of tissues for biomedical applications (in relation to tissues of the face and body, cartilage, skin, and hair).

Basic research related to tissue regeneration, both in vitro and in vivo, will definitively contribute to the regenerative plastic surgery field.

In light of current knowledge on chondrocytes, epithelial and dermal cells, human follicle cells (HFSCs), and adipose-derived stem cells (ASCs) and their associations with biomaterials, numerous researchers have developed different strategies to improve the effects of these varied biotechnology applications.

This Special Issue aims to collect submissions of original research articles and reviews that demonstrate biomolecular pathways and effects of stem cells, PRP, micrografts, and biomaterials in regenerative plastic surgery.

Potential topics include, but are not limited to, the following:

Breast reconstruction with hybrid techniques, represented by prosthesis and biomaterials (mesh), analyzed by instrumental and clinical evaluations in patients with breast cancer and mastectomy outcomes;
Hair regrowth estimated by TrichoScan, hair dystrophy estimated by dermoscopy, and cell proliferation estimated by Ki67 assessment in patients affected by hair loss treated with micrografts, cells (follicular/ASC/SVF/epithelial/dermal), PRP, growth factors, and other biotechnologies;
Fat graft maintenance estimated by instrumental evaluation in patients with face and breast soft tissue defects treated with fat injections enriched with stem cells (ASC/SVF) and/or PRP;
Cartilage and chondrocytes in nose reconstruction estimated by instrumental and team evaluations in patients with minor soft tissue defects of the nose (valve collapse and pinch nose deformity) treated with cartilage paste grafting (diced cartilage, shaving cartilage, and crushing) and/or chondrocytes; and
Ulcer treatment performed with biomaterials/PRP/stem cells, estimated by instrumental and team evaluations.

Dr. Pietro Gentile
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

regenerative medicine
regenerative plastic surgery
adipose-derived stem cells
platelet-rich plasma
biomaterials
micrografts

Published Papers (2 papers)

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Research

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9 pages, 238 KiB

Open AccessArticle

Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

by Pietro Gentile, Claudio Calabrese, Barbara De Angelis, Laura Dionisi, Jacopo Pizzicannella, Ashutosh Kothari, Domenico De Fazio and Simone Garcovich

Int. J. Mol. Sci. 2020, 21(2), 431; https://doi.org/10.3390/ijms21020431 - 9 Jan 2020

Cited by 80 | Viewed by 7670

Abstract

Autologous therapies using platelet-rich plasma (PRP) need meticulous preparation—currently, no standardised preparation technique exists. Processing Quantitative Standards (PQSs) define manufacturing quantitative variables (such as time, volume and pressure). Processing Qualitative Standards (PQLSs) define the quality of the materials and methods of manufacturing. The aim of this review is to use existing PQSs and PQLs to report the in vivo/in vitro results obtained by using different Kits, that utilise different procedures (classified as Closed-Technique and Opened-Technique) to isolate autologous human activated (AA-PRP) or non-activated PRP (A-PRP). PQSs included the volumes of blood collected as well as the reagents used, the time/gravity of centrifugation, and the duration, temperature and tilt level/speed of centrifugation. PQLSs included the use of Calcium Chloride CaCl_2, Kit weight, transparency of Kit components, the maintenance of a closed sterile processing environment and the use of a small centrifuge. Eight CE marked devices for PRP extraction were evaluated: Angel^®, Biomed^®, Cascade^® and Selphyl^®, Mag-18^®, i-Stem^®, MyCells^® and Regenlab^®. Using a Kit with the PQSs and PQLSs described in this study enables the isolation of A-PRP, thereby meeting consensus quality criteria. As our understanding of Critical Quality Attributes (CQAs) of A-PRP continues to evolve, especially with respect to purity and potency, adjustments to these benchmark PQSs and PQLs will hopefully help isolate A-PRP of desired CQAs with greater reproducibility, quality, and safety. Confirmatory studies will no doubt need to be completed. Full article

(This article belongs to the Special Issue Regenerative Applications of Stem Cells, PRP, Micrografts, and Biomaterials)

Review

Jump to: Research

26 pages, 324 KiB

Open AccessReview

Impact of the Different Preparation Methods to Obtain Human Adipose-Derived Stromal Vascular Fraction Cells (AD-SVFs) and Human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs): Enzymatic Digestion Versus Mechanical Centrifugation

by Pietro Gentile, Claudio Calabrese, Barbara De Angelis, Jacopo Pizzicannella, Ashutosh Kothari and Simone Garcovich

Int. J. Mol. Sci. 2019, 20(21), 5471; https://doi.org/10.3390/ijms20215471 - 2 Nov 2019

Cited by 77 | Viewed by 5973

Abstract

Autologous therapies using adipose-derived stromal vascular fraction (AD-SVFs) and adult adipose-derived mesenchymal stem cells (AD-MSCs) warrant careful preparation of the harvested adipose tissue. Currently, no standardized technique for this preparation exists. Processing quantitative standards (PQSs) define manufacturing quantitative variables (such as time, volume, and pressure). Processing qualitative standards (PQLSs) define the quality of the materials and methods in manufacturing. The purpose of the review was to use PQSs and PQLSs to report the in vivo and in vitro results obtained by different processing kits that use different procedures (enzymatic vs. non-enzymatic) to isolate human AD-SVFs/AD-MSCs. PQSs included the volume of fat tissue harvested and reagents used, the time/gravity of centrifugation, and the time, temperature, and tilt level/speed of incubation and/or centrifugation. PQLSs included the use of a collagenase, a processing time of 30 min, kit weight, transparency of the kit components, the maintenance of a closed sterile processing environment, and the use of a small centrifuge and incubating rocker. Using a kit with the PQSs and PQLSs described in this study enables the isolation of AD-MSCs that meet the consensus quality criteria. As the discovery of new critical quality attributes (CQAs) of AD-MSCs evolve with respect to purity and potency, adjustments to these benchmark PQSs and PQLs will hopefully isolate AD-MSCs of various CQAs with greater reproducibility, quality, and safety. Confirmatory studies will no doubt need to be completed. Full article

(This article belongs to the Special Issue Regenerative Applications of Stem Cells, PRP, Micrografts, and Biomaterials)

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