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Molecular Research Based on Flow Cytometry
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Molecular Research Based on Flow Cytometry

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: closed (31 March 2023) | Viewed by 4797

Special Issue Editors

Dr. Paola Lanuti

E-Mail Website
Guest Editor
Department of Medicine and Aging Science, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, Italy
Interests: extracellular vesicles; flow cytometry; biomarkers
Special Issues, Collections and Topics in MDPI journals
Dr. Pasquale Simeone

E-Mail Website
Guest Editor
Center for Advanced Studies and Technology (CAST), Laboratory of Cytomorphology, Department of Medicine and Aging Sciences, University "G. 'Annunzio" - Chieti, Via Luigi Polacchi 11-13, 66100 Chieti Scalo, Italy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Flow cytometry is an emerging technique, characterized by the possibility of analyzing a panel of antigens and/or cell functions at a single-cell level. Flow cytometry has been also identified as a highly powerful technology to investigate the ability of cells to communicate via extracellular vesicles.

Recently, advancements in related disciplines (i.e., multispectral technology, enhanced instrument performance) have increased its polychromatic potential, making it theoretically possible to analyze more than 30 parameters at the same time.

This Special Issue aims to underline the possibility of using flow cytometry in “from bench to bed” applications. In detail, it will focus on basic research flow cytometry findings, as well as on the possibility of translating new protocols or new applications into clinical practice, with attention paid to the immunology and hematology fields.

Reviews, new flow-cytometry-based protocols, original papers and short communications based on different flow cytometry applications will be considered for publication in this Special Issue.

Dr. Paola Lanuti
Dr. Pasquale Simeone
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Flow Cytometry
  • Extracellular Vesicles
  • Hematological diseases
  • Immunology
  • Oncology
  • Genetic Disorders

Published Papers (2 papers)

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Research

20 pages, 5126 KiB  
Article
Imaging Flow Cytometry Demonstrates Physiological and Morphological Diversity within Treated Probiotic Bacteria Groups
by Jakub Kiepś, Wojciech Juzwa and Radosław Dembczyński
Int. J. Mol. Sci. 2023, 24(7), 6841; https://doi.org/10.3390/ijms24076841 - 6 Apr 2023
Cited by 4 | Viewed by 1914
Abstract
Probiotic bacteria can be introduced to stresses during the culturing phase as an alternative to the use of protectants and coating substances during drying. Accurate enumeration of the bacterial count in a probiotic formulation can be provided using imaging flow cytometry (IFC). IFC [...] Read more.
Probiotic bacteria can be introduced to stresses during the culturing phase as an alternative to the use of protectants and coating substances during drying. Accurate enumeration of the bacterial count in a probiotic formulation can be provided using imaging flow cytometry (IFC). IFC overcomes the weak points of conventional, commonly used flow cytometry by combining its statistical power with the imaging content of microscopy in one system. Traditional flow cytometers only collect the fluorescence signal intensities, while IFC provides many more steps as it correlates the data on the measured parameters of fluorescence light with digitally processed images of the analyzed cells. As an alternative to standard methods (plate cell counts and traditional flow cytometry) IFC provides additional insight into the physiology and morphology of the cell. The use of complementary dyes (RedoxSensorTM Green and propidium iodide) allows for the designation of groups based on their metabolic activity and membrane damage. Additionally, cell sorting is incorporated to assess each group in terms of growth on different media (MRS-Agar and MRS broth). Results show that the groups with intermediate metabolic activity and some degree of cellular damage correspond with the description of viable but nonculturable cells. Full article
(This article belongs to the Special Issue Molecular Research Based on Flow Cytometry)
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9 pages, 926 KiB  
Communication
Basophil Activation Test with Different Polyethylene Glycols in Patients with Suspected PEG Hypersensitivity Reactions
by Simone Vespa, Pietro Del Biondo, Pasquale Simeone, Enrico Cavallucci, Giulia Catitti, Raffaella Auciello, Domenico De Bellis, Isotta Altomare, Laura Pierdomenico, Barbara Canonico, Ilaria Cicalini, Ilaria Angilletta, Piero Del Boccio, Damiana Pieragostino, Francesca Santilli, Andrea Urbani, Vincenzo De Laurenzi, Liborio Stuppia and Paola Lanuti
Int. J. Mol. Sci. 2022, 23(23), 14592; https://doi.org/10.3390/ijms232314592 - 23 Nov 2022
Cited by 5 | Viewed by 1379
Abstract
Allergic reactions to COVID-19 vaccine components are rare but should be considered. Polyethylene glycol (PEG) is responsible for anaphylaxis in mRNA vaccines. Skin tests have been used in the allergological work-up programs for COVID-19 vaccine evaluation. However, the reproducibility of the skin prick [...] Read more.
Allergic reactions to COVID-19 vaccine components are rare but should be considered. Polyethylene glycol (PEG) is responsible for anaphylaxis in mRNA vaccines. Skin tests have been used in the allergological work-up programs for COVID-19 vaccine evaluation. However, the reproducibility of the skin prick test is time-dependent and the reactivity declines over time. Therefore, we combined the administration of the skin tests with the basophil activation test (BAT) using PEG2000, PEG4000 and DMG-PEG2000, where the BAT was considered positive when the percentage of activated basophils was higher than 6%, 5% and 6.5%, for PEG 4000, PEG2000 and DMG-PEG2000, respectively. To this end, among the subjects that underwent allergy counseling at the Allergy Unit of our Institution during the 2020/2021 vaccination campaign, 13 patients had a suggested medical history of PEG/drug hypersensitivity and were enrolled together with 10 healthy donors. Among the enrolled patients 2 out of 13 tested patients were positive to the skin test. The BAT was negative in terms of the percentages of activated basophils in all analyzed samples, but the stimulation index (SI) was higher than 2.5 in 4 out of 13 patients. These data evidenced that, when the SI is higher than 2.5, even in the absence of positivity to BAT, the BAT to PEG may be a useful tool to be coupled to skin tests to evidence even low-grade reactions. Full article
(This article belongs to the Special Issue Molecular Research Based on Flow Cytometry)
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