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Biosensors
Special Issues
Biosensors Based on Isothermal Nucleic Acid Amplification Strategies
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Biosensors Based on Isothermal Nucleic Acid Amplification Strategies

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: 30 May 2024 | Viewed by 1486

Special Issue Editors

Dr. Yongxin Li

E-Mail Website
Guest Editor
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China
Interests: fluorescence biosensor; fiber optic biosensor; isothermal nucleic acid amplification; nanomaterials; public health; pathogenic bacteria; virus
Dr. Zewei Luo

E-Mail Website
Guest Editor
Research Center of Analytical Instrumentation, School of Mechanical Engineering, Sichuan University, Chengdu 610041, China
Interests: fiber optic-based biosensor; isothermal nucleic acid amplification; nanomaterials; aptamer; public health; environmental monitoring; clinic diagnosis; food safety
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue focuses on biosensors based on isothermal nucleic acid amplification strategies for food safety, environmental monitoring, and clinical diagnostic research. Isothermal nucleic acid amplification does not require expensive variable temperature equipment, can be performed under isothermal conditions or at room temperature, and is suitable for rapid, in situ analysis and point-of-care testing. To this end, the purpose of this Special Issue is to collect original papers and reviews to show the development of isothermal nucleic acid amplification strategy-based biosensors and their innovative applications in related fields, new challenges and development prospects.

Dr. Yongxin Li
Dr. Zewei Luo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • isothermal nucleic acid amplification
  • nanomaterials
  • aptamer
  • public health
  • environmental monitoring
  • clinic diagnosis
  • food safety

Published Papers (1 paper)

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Research

13 pages, 3576 KiB  
Article
Low-Cost Arduino Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) for Sensitive Nucleic Acid Detection
by Bruno Dias Camargo, Mateus Cassaboni Stracke, Heloisa Bruna Soligo Sanchuki, Viviane Klassen de Oliveira, Hellen Cristina Ancelmo, Dayanne Mozaner Bordin, Fabricio Klerynton Marchini, Emilson Ribeiro Viana and Lucas Blanes
Biosensors 2024, 14(3), 128; https://doi.org/10.3390/bios14030128 - 29 Feb 2024
Viewed by 1228
Abstract
This work presents a low-cost transcription loop-mediated isothermal amplification (RT-LAMP) instrument for nucleic acid detection, employing an Arduino Nano microcontroller. The cooling system includes customized printed circuit boards (PCBs) that serve as electrical resistors and incorporate fans. An aluminum block is designed to [...] Read more.
This work presents a low-cost transcription loop-mediated isothermal amplification (RT-LAMP) instrument for nucleic acid detection, employing an Arduino Nano microcontroller. The cooling system includes customized printed circuit boards (PCBs) that serve as electrical resistors and incorporate fans. An aluminum block is designed to accommodate eight vials. The system also includes two PCB heaters—one for sample heating and the other for vial lid heating to prevent condensation. The color detection system comprises a TCS3200 color 8-sensor array coupled to one side of the aluminum heater body and a white 8-LED array coupled to the other side, controlled by two Multiplexer/Demultiplexer devices. LED light passes through the sample, reaching the color sensor and conveying color information crucial for detection. The top board is maintained at 110 ± 2 °C, while the bottom board is held at 65 ± 0.5 °C throughout the RT-LAMP assay. Validation tests successfully demonstrated the efficacy of the colorimetric RT-LAMP reactions using SARS-CoV-2 RNA amplification as a sample viability test, achieving 100% sensitivity and 97.3% specificity with 66 clinical samples. Our instrument offers a cost-effective (USD 100) solution with automated result interpretation and superior sensitivity compared to visual inspection. While the prototype was tested with SARS-CoV-2 RNA samples, its versatility extends to detecting other pathogens using alternative primers, showcasing its potential for broader applications in biosensing. Full article
(This article belongs to the Special Issue Biosensors Based on Isothermal Nucleic Acid Amplification Strategies)
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