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Journals
Agronomy
Special Issues
Modern In Vitro Technologies for Developing Horticulture
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Modern In Vitro Technologies for Developing Horticulture

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Horticultural and Floricultural Crops".

Deadline for manuscript submissions: 31 October 2024 | Viewed by 473

Special Issue Editor

Dr. Matteo Caser

E-Mail Website
Guest Editor
Department of Agricultural, Forest, and Food Sciences, DISAFA, Ornamental Crops and Landscape Design, University of Turin, 10095 Grugliasco, Italy
Interests: micropropagation; horticulture; floriculture; medicinal and aromatic plants; sustainable cultivation; soilless cultivation; abiotic stress; secondary metabolites; biostimulants
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Horticultural businesses, including those that sell ornamental and medicinal plants, always seek new trends and niches to increase product sales. Innovative in vitro techniques are constantly being developed via academic research and experimental trials. This Special Issue aims to record the most recent and novel findings for the development of micropropagation technologies for developing the horticultural sector, with particular interest in sustainable production, biodiversity conservation, and plant protection.

Dr. Matteo Caser
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • ornamentals
  • horticulture
  • medicinal plants
  • bioreactor
  • stress tolerance
  • cyropreservation genetic modification
  • micropropagation
  • nanotechnology
  • bioeconomy
  • genomics
  • somaclonal variability
  • omics
  • genetic engineering
  • in vitro
  • stress resilience
  • endangered species
  • rare species
  • biodiversity

Published Papers (1 paper)

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Research

16 pages, 4307 KiB  
Article
Micropropagation of Al-Taif Rose: Effects of Medium Constituents and Light on In Vitro Rooting and Acclimatization
by Ali Mohsen Al-Ali, Yaser Hassan Dewir and Rashid Sultan Al-Obeed
Agronomy 2024, 14(6), 1120; https://doi.org/10.3390/agronomy14061120 - 24 May 2024
Viewed by 222
Abstract
Micropropagation facilitates the rapid production of roses. Nevertheless, in vitro rhizogenesis of essential oil roses has presented significant challenges, primarily attributed to low rates of rooting and poorer acclimatization compared to ornamental rose varieties. This study reports the optimization of in vitro rooting [...] Read more.
Micropropagation facilitates the rapid production of roses. Nevertheless, in vitro rhizogenesis of essential oil roses has presented significant challenges, primarily attributed to low rates of rooting and poorer acclimatization compared to ornamental rose varieties. This study reports the optimization of in vitro rooting of Al-Taif rose (Rosa damascena f. trigintipetala (Diek) R. Keller) microshoots with the aim of increasing survival rate during acclimatization. We also investigated the effects of various parameters, including type and concentration of auxin (i.e., 2,4-Dichlorophenoxyacetic acid (2,4-D), indole acetic acid (IAA), indole butyric acid (IBA), and naphthaleneacetic acid (NAA) at concentrations of 0, 0.05, 0.1, 0.2, and 0.4 mg/L), salt strength (i.e., full- and half-strength Murashige and Skoog (MS) medium), sucrose concentration (i.e., 20, 30, 40, 60, and 80 g/L), light spectra (a 2:1 or 1:2 blue/red spectral ratio, cool or warm white light at a 1:1 ratio, and fluorescent light), light intensity (photosynthetic photon flux density (PPFD) values of 25, 50, and 100 µmol·m−2·s−1), and activated charcoal (i.e., 0 and 0.5 g/L) on the rooting and growth of in vitro regenerated Al-Taif rose axillary shoots. We found that half-strength MS medium supplemented with 0.2 mg/L NAA, 80 g/L sucrose, 0.5 g/L activated charcoal, and 50 μmol·m−2·s−1 PPFD were the optimal conditions for 100% induction of adventitious roots. Next, micropropagated Al-Taif rose plantlets were successfully transferred to a potting medium containing perlite/peatmoss (in a 1:1 ratio). We found that 98% of plants survived ex vitro conditions. The genetic fidelity of micropropagated Al-Taif rose clones along with their mother plant was tested using the inter-simple sequence repeats (ISSR) molecular marker. The genetic similarity between the micropropagated plantlets and the mother plant of Al-Taif rose plants was 98.8%, revealing high uniformity and true-to-type regenerated plants. These findings may therefore contribute toward the commercial micropropagation of Al-Taif roses. Full article
(This article belongs to the Special Issue Modern In Vitro Technologies for Developing Horticulture)
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