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Processes 2014, 2(1), 34-57; doi:10.3390/pr2010034
Article

Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications

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Received: 18 September 2013; in revised form: 5 December 2013 / Accepted: 19 December 2013 / Published: 3 January 2014
(This article belongs to the Special Issue Design of Bioreactor Systems for Tissue Engineering)
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Abstract: Radial flow perfusion of cell-seeded hollow cylindrical porous scaffolds may overcome the transport limitations of pure diffusion and direct axial perfusion in the realization of bioengineered substitutes of failing or missing tissues. Little has been reported on the optimization criteria of such bioreactors. A steady-state model was developed, combining convective and dispersive transport of dissolved oxygen with Michaelis-Menten cellular consumption kinetics. Dimensional analysis was used to combine more effectively geometric and operational variables in the dimensionless groups determining bioreactor performance. The effectiveness of cell oxygenation was expressed in terms of non-hypoxic fractional construct volume. The model permits the optimization of the geometry of hollow cylindrical constructs, and direction and magnitude of perfusion flow, to ensure cell oxygenation and culture at controlled oxygen concentration profiles. This may help engineer tissues suitable for therapeutic and drug screening purposes.
Keywords: bioreactor; model; oxygen; radial flow; tissue engineering; transport bioreactor; model; oxygen; radial flow; tissue engineering; transport
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Donato, D.; Napoli, I.E.D.; Catapano, G. Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications. Processes 2014, 2, 34-57.

AMA Style

Donato D, Napoli IED, Catapano G. Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications. Processes. 2014; 2(1):34-57.

Chicago/Turabian Style

Donato, Danilo; Napoli, Ilaria E.D.; Catapano, Gerardo. 2014. "Model-Based Optimization of Scaffold Geometry and Operating Conditions of Radial Flow Packed-Bed Bioreactors for Therapeutic Applications." Processes 2, no. 1: 34-57.

Processes EISSN 2227-9717 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert