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Processes 2014, 2(1), 71-88; doi:10.3390/pr2010071

A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

1,* , 2,†
1 Communication Sciences and Disorders, the University of Iowa, Iowa City, IA 52242, USA 2 Department of Biomedical Engineering, the University of Iowa, Iowa City, IA 52242, USA 3 National Center for Voice and Speech, University of Utah, Salt Lake City, UT 84112, USA These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 13 September 2013 / Revised: 25 November 2013 / Accepted: 20 December 2013 / Published: 10 January 2014
(This article belongs to the Special Issue Design of Bioreactor Systems for Tissue Engineering)
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To mimic in vivo vibration of vocal fold cells, we studied the controllability and range of frequency, acceleration, duration, and shear stress in a new bioreactor attachment. The custom multiwell disc appliance fits into a commercially built rheometer, together termed a torsional rheometer bioreactor (TRB). Previous attachments to the TRB were capable of 50–100 Hz vibrations at relatively high strains but were limited to single-sample experiments. The TRB-multiwell disc system accommodates 20 samples in partially fluid-filled wells in an aseptic environment delivering three different acceleration conditions to different samples simultaneously. Frequency and amplitude used to calculate acceleration along with duration and shear stress were controllable and quantifiable using a combination of built-in rheometer sensors, manufacturer software, and smooth particle hydrodynamics (SPH) simulations. Computed shear stresses at the well bottom using SPH in two and three dimensions were verified with analytical approximations. Results demonstrate capabilities of the TRB-multiwell disc system that, when combined with computational modeling, provide quantifiable vibration parameters covering frequencies 0.01–250 Hz, accelerations of 0.02–300 m/s2, and shear stresses of 0.01–1.4 Pa. It is well-suited for studying cell function underlying vocal fold lamina propria homeostasis, inflammation, and wound healing under differential vibration conditions.
Keywords: bioreactor; vocal fold; vibration; smooth particle hydrodynamics; rheometer bioreactor; vocal fold; vibration; smooth particle hydrodynamics; rheometer
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Klemuk, S.A.; Vigmostad, S.; Endapally, K.; Wagner, A.P.; Titze, I.R. A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies. Processes 2014, 2, 71-88.

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