Processes 2014, 2(1), 71-88; doi:10.3390/pr2010071
Article

A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies

Received: 13 September 2013; in revised form: 25 November 2013 / Accepted: 20 December 2013 / Published: 10 January 2014
(This article belongs to the Special Issue Design of Bioreactor Systems for Tissue Engineering)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: To mimic in vivo vibration of vocal fold cells, we studied the controllability and range of frequency, acceleration, duration, and shear stress in a new bioreactor attachment. The custom multiwell disc appliance fits into a commercially built rheometer, together termed a torsional rheometer bioreactor (TRB). Previous attachments to the TRB were capable of 50–100 Hz vibrations at relatively high strains but were limited to single-sample experiments. The TRB-multiwell disc system accommodates 20 samples in partially fluid-filled wells in an aseptic environment delivering three different acceleration conditions to different samples simultaneously. Frequency and amplitude used to calculate acceleration along with duration and shear stress were controllable and quantifiable using a combination of built-in rheometer sensors, manufacturer software, and smooth particle hydrodynamics (SPH) simulations. Computed shear stresses at the well bottom using SPH in two and three dimensions were verified with analytical approximations. Results demonstrate capabilities of the TRB-multiwell disc system that, when combined with computational modeling, provide quantifiable vibration parameters covering frequencies 0.01–250 Hz, accelerations of 0.02–300 m/s2, and shear stresses of 0.01–1.4 Pa. It is well-suited for studying cell function underlying vocal fold lamina propria homeostasis, inflammation, and wound healing under differential vibration conditions.
Keywords: bioreactor; vocal fold; vibration; smooth particle hydrodynamics; rheometer
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MDPI and ACS Style

Klemuk, S.A.; Vigmostad, S.; Endapally, K.; Wagner, A.P.; Titze, I.R. A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies. Processes 2014, 2, 71-88.

AMA Style

Klemuk SA, Vigmostad S, Endapally K, Wagner AP, Titze IR. A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies. Processes. 2014; 2(1):71-88.

Chicago/Turabian Style

Klemuk, Sarah A.; Vigmostad, Sarah; Endapally, Kalyan; Wagner, Andrew P.; Titze, Ingo R. 2014. "A Multiwell Disc Appliance Used to Deliver Quantifiable Accelerations and Shear Stresses at Sonic Frequencies." Processes 2, no. 1: 71-88.

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