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Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus
Healthcare Engineering Research Group, Centre for Biological Engineering, Loughborough University, Loughborough LE11 3TU, UK
ElectroForce Systems Group, Bose Corporation, Eden Prairie, MN 55346, USA
* Authors to whom correspondence should be addressed.
Received: 30 September 2013; in revised form: 20 November 2013 / Accepted: 12 December 2013 / Published: 20 December 2013
Abstract: A novel multi-axial bioreactor was designed and developed to deliver combinations of the following dynamic mechanical stimulation conditions: hydrostatic pressure, pulsatile perfusion flow and uniaxial compression in order to mimic in vivo conditions. This mechanical arrangement simultaneously allows triaxial stimulation and characterization of mechanical properties of samples, in particular simulating the conditions experienced by the nucleus pulposus in vivo. A series of initial experiments were performed on this prototype system using consistent, commercially-available, three dimensional scaffolds in combination with human dermal fibroblasts. Our results show that while such bioreactors hold much promise in tissue engineering of desired organs, achieving the right combination of mechanical stimuli and other conditions required in order to enhance the final properties of the cell-scaffold systems is challenging.
Keywords: bioreactor; tissue engineering; nucleus pulposus; mechanical stimulation
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MDPI and ACS Style
Naing, M.W.; Liu, Y.; Sebastine, I.; Dingmann, D.; Williams, C.; Williams, D.J. Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus. Processes 2014, 2, 1-11.
Naing MW, Liu Y, Sebastine I, Dingmann D, Williams C, Williams DJ. Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus. Processes. 2014; 2(1):1-11.
Naing, May W.; Liu, Yang; Sebastine, Immanuel; Dingmann, David; Williams, Chrysanthi; Williams, David J. 2014. "Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus." Processes 2, no. 1: 1-11.