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Processes 2014, 2(1), 1-11; doi:10.3390/pr2010001
Technical Note

Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus

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Received: 30 September 2013; in revised form: 20 November 2013 / Accepted: 12 December 2013 / Published: 20 December 2013
(This article belongs to the Special Issue Design of Bioreactor Systems for Tissue Engineering)
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Abstract: A novel multi-axial bioreactor was designed and developed to deliver combinations of the following dynamic mechanical stimulation conditions: hydrostatic pressure, pulsatile perfusion flow and uniaxial compression in order to mimic in vivo conditions. This mechanical arrangement simultaneously allows triaxial stimulation and characterization of mechanical properties of samples, in particular simulating the conditions experienced by the nucleus pulposus in vivo. A series of initial experiments were performed on this prototype system using consistent, commercially-available, three dimensional scaffolds in combination with human dermal fibroblasts. Our results show that while such bioreactors hold much promise in tissue engineering of desired organs, achieving the right combination of mechanical stimuli and other conditions required in order to enhance the final properties of the cell-scaffold systems is challenging.
Keywords: bioreactor; tissue engineering; nucleus pulposus; mechanical stimulation bioreactor; tissue engineering; nucleus pulposus; mechanical stimulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Naing, M.W.; Liu, Y.; Sebastine, I.; Dingmann, D.; Williams, C.; Williams, D.J. Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus. Processes 2014, 2, 1-11.

AMA Style

Naing MW, Liu Y, Sebastine I, Dingmann D, Williams C, Williams DJ. Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus. Processes. 2014; 2(1):1-11.

Chicago/Turabian Style

Naing, May W.; Liu, Yang; Sebastine, Immanuel; Dingmann, David; Williams, Chrysanthi; Williams, David J. 2014. "Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus." Processes 2, no. 1: 1-11.

Processes EISSN 2227-9717 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert