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Processes 2014, 2(1), 1-11;

Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus

Healthcare Engineering Research Group, Centre for Biological Engineering, Loughborough University, Loughborough LE11 3TU, UK
ElectroForce Systems Group, Bose Corporation, Eden Prairie, MN 55346, USA
Authors to whom correspondence should be addressed.
Received: 30 September 2013 / Revised: 20 November 2013 / Accepted: 12 December 2013 / Published: 20 December 2013
(This article belongs to the Special Issue Design of Bioreactor Systems for Tissue Engineering)
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A novel multi-axial bioreactor was designed and developed to deliver combinations of the following dynamic mechanical stimulation conditions: hydrostatic pressure, pulsatile perfusion flow and uniaxial compression in order to mimic in vivo conditions. This mechanical arrangement simultaneously allows triaxial stimulation and characterization of mechanical properties of samples, in particular simulating the conditions experienced by the nucleus pulposus in vivo. A series of initial experiments were performed on this prototype system using consistent, commercially-available, three dimensional scaffolds in combination with human dermal fibroblasts. Our results show that while such bioreactors hold much promise in tissue engineering of desired organs, achieving the right combination of mechanical stimuli and other conditions required in order to enhance the final properties of the cell-scaffold systems is challenging. View Full-Text
Keywords: bioreactor; tissue engineering; nucleus pulposus; mechanical stimulation bioreactor; tissue engineering; nucleus pulposus; mechanical stimulation

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Naing, M.W.; Liu, Y.; Sebastine, I.; Dingmann, D.; Williams, C.; Williams, D.J. Design and Validation of a Physiologically-Adapted Bioreactor for Tissue Engineering of the Nucleus Pulposus. Processes 2014, 2, 1-11.

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