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J. Funct. Biomater. 2012, 3(1), 183-198; doi:10.3390/jfb3010183
Article

Experimental Models for Investigating Intra-Stromal Migration of Corneal Keratocytes, Fibroblasts and Myofibroblasts

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Received: 27 January 2012; in revised form: 10 March 2012 / Accepted: 13 March 2012 / Published: 19 March 2012
(This article belongs to the Special Issue Corneal Scarring: Wound Healing and Biomaterials)
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Abstract: Following laser vision correction, corneal keratocytes must repopulate areas of cell loss by migrating through the intact corneal stroma, and this can impact corneal shape and transparency. In this study, we evaluate 3D culture models for simulating this process in vitro. Buttons (8 mm diameter) were first punched out of keratocyte populated compressed collagen matrices, exposed to a 3 mm diameter freeze injury, and cultured in serum-free media (basal media) or media supplemented with 10% FBS, TGFb1 or PDGF BB. Following freeze injury, a region of cell death was observed in the center of the constructs. Although cells readily migrated on top of the matrices to cover the wound area, a limited amount of cell migration was observed within the constructs. We next developed a novel “sandwich” model, which better mimics the native lamellar architecture of the cornea. Using this model, significant migration was observed under all conditions studied. In both models, cells in TGFb and 10% FBS developed stress fibers; whereas cells in PDGF were more dendritic. PDGF stimulated the most inter-lamellar migration in the sandwich construct. Overall, these models provide insights into the complex interplay between growth factors, cell mechanical phenotypes and the structural properties of the ECM.
Keywords: extracellular matrix; corneal keratocytes; cell mechanics; growth factors; 3D culture extracellular matrix; corneal keratocytes; cell mechanics; growth factors; 3D culture
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Petroll, W.M.; Lakshman, N.; Ma, L. Experimental Models for Investigating Intra-Stromal Migration of Corneal Keratocytes, Fibroblasts and Myofibroblasts. J. Funct. Biomater. 2012, 3, 183-198.

AMA Style

Petroll WM, Lakshman N, Ma L. Experimental Models for Investigating Intra-Stromal Migration of Corneal Keratocytes, Fibroblasts and Myofibroblasts. Journal of Functional Biomaterials. 2012; 3(1):183-198.

Chicago/Turabian Style

Petroll, Walter Matthew; Lakshman, Neema; Ma, Lisha. 2012. "Experimental Models for Investigating Intra-Stromal Migration of Corneal Keratocytes, Fibroblasts and Myofibroblasts." J. Funct. Biomater. 3, no. 1: 183-198.



J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert