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Sustained Delivery of Chondroitinase ABC from Hydrogel System
Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”, Politecnico di Milano, via Mancinelli 7, Milan 20131, Italy
Department of Neuroscience, Mario Negri Institute for Pharmacological Research, via La Masa 19, Milan 20156, Italy
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 19 December 2011; in revised form: 27 February 2012 / Accepted: 13 March 2012 / Published: 19 March 2012
Abstract: In the injured spinal cord, chondroitin sulfate proteoglycans (CSPGs) are the principal responsible of axon growth inhibition and they contribute to regenerative failure, promoting glial scar formation. Chondroitinase ABC (chABC) is known for being able to digest proteoglycans, thus degrading glial scar and favoring axonal regrowth. However, its classic administration is invasive, infection-prone and clinically problematic. An agarose-carbomer (AC1) hydrogel, already used in SCI repair strategies, was here investigated as a delivery system capable of an effective chABC administration: the material ability to include chABC within its pores and the possibility to be injected into the target tissue were firstly proved. Subsequently, release kinetic and the maintenance of enzymatic activity were positively assessed: AC1 hydrogel was thus confirmed to be a feasible tool for chABC delivery and a promising device for spinal cord injury topic repair strategies.
Keywords: chondroitinase ABC; hydrogels; drug delivery; spinal cord injury
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Rossi, F.; Veglianese, P.; Santoro, M.; Papa, S.; Rogora, C.; Dell’Oro, V.; Forloni, G.; Masi, M.; Perale, G. Sustained Delivery of Chondroitinase ABC from Hydrogel System. J. Funct. Biomater. 2012, 3, 199-208.
Rossi F, Veglianese P, Santoro M, Papa S, Rogora C, Dell’Oro V, Forloni G, Masi M, Perale G. Sustained Delivery of Chondroitinase ABC from Hydrogel System. Journal of Functional Biomaterials. 2012; 3(1):199-208.
Rossi, Filippo; Veglianese, Pietro; Santoro, Marco; Papa, Simonetta; Rogora, Cristina; Dell’Oro, Valentina; Forloni, Gianluigi; Masi, Maurizio; Perale, Giuseppe. 2012. "Sustained Delivery of Chondroitinase ABC from Hydrogel System." J. Funct. Biomater. 3, no. 1: 199-208.