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J. Funct. Biomater. 2012, 3(1), 173-182; doi:10.3390/jfb3010173
Communication

Gas-Foamed Scaffold Gradients for Combinatorial Screening in 3D

1,2
, 1
, 3
, 3
 and Jr. 1,*
Received: 6 February 2012; in revised form: 28 February 2012 / Accepted: 1 March 2012 / Published: 7 March 2012
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Abstract: Current methods for screening cell-material interactions typically utilize a two-dimensional (2D) culture format where cells are cultured on flat surfaces. However, there is a need for combinatorial and high-throughput screening methods to systematically screen cell-biomaterial interactions in three-dimensional (3D) tissue scaffolds for tissue engineering. Previously, we developed a two-syringe pump approach for making 3D scaffold gradients for use in combinatorial screening of salt-leached scaffolds. Herein, we demonstrate that the two-syringe pump approach can also be used to create scaffold gradients using a gas-foaming approach. Macroporous foams prepared by a gas-foaming technique are commonly used for fabrication of tissue engineering scaffolds due to their high interconnectivity and good mechanical properties. Gas-foamed scaffold gradient libraries were fabricated from two biodegradable tyrosine-derived polycarbonates: poly(desaminotyrosyl-tyrosine ethyl ester carbonate) (pDTEc) and poly(desaminotyrosyl-tyrosine octyl ester carbonate) (pDTOc). The composition of the libraries was assessed with Fourier transform infrared spectroscopy (FTIR) and showed that pDTEc/pDTOc gas-foamed scaffold gradients could be repeatably fabricated. Scanning electron microscopy showed that scaffold morphology was similar between the pDTEc-rich ends and the pDTOc-rich ends of the gradient. These results introduce a method for fabricating gas-foamed polymer scaffold gradients that can be used for combinatorial screening of cell-material interactions in 3D.
Keywords: combinatorial screening; polymer; scaffold; tissue engineering combinatorial screening; polymer; scaffold; tissue engineering
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chatterjee, K.; Kraigsley, A.M.; Bolikal, D.; Kohn, J.; Simon, C.G., Jr. Gas-Foamed Scaffold Gradients for Combinatorial Screening in 3D. J. Funct. Biomater. 2012, 3, 173-182.

AMA Style

Chatterjee K, Kraigsley AM, Bolikal D, Kohn J, Simon CG, Jr. Gas-Foamed Scaffold Gradients for Combinatorial Screening in 3D. Journal of Functional Biomaterials. 2012; 3(1):173-182.

Chicago/Turabian Style

Chatterjee, Kaushik; Kraigsley, Alison M.; Bolikal, Durgadas; Kohn, Joachim; Simon, Carl G., Jr. 2012. "Gas-Foamed Scaffold Gradients for Combinatorial Screening in 3D." J. Funct. Biomater. 3, no. 1: 173-182.



J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert