Next Article in Journal
Experimental Models for Investigating Intra-Stromal Migration of Corneal Keratocytes, Fibroblasts and Myofibroblasts
Previous Article in Journal
Development of Ti-Coated Ferromagnetic Needle, Adaptable for Ablation Cancer Therapy by High-Frequency Induction Heating
Article Menu

Export Article

Open AccessCommunication
J. Funct. Biomater. 2012, 3(1), 173-182; doi:10.3390/jfb3010173

Gas-Foamed Scaffold Gradients for Combinatorial Screening in 3D

1
Polymers Division, National Institute of Standards & Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA
2
Department of Materials Engineering, Indian Institute of Science, Bangalore 560012, India
3
New Jersey Center for Biomaterials, Rutgers University, Piscataway, NJ 08854, USA
*
Author to whom correspondence should be addressed.
Received: 6 February 2012 / Revised: 28 February 2012 / Accepted: 1 March 2012 / Published: 7 March 2012
View Full-Text   |   Download PDF [1059 KB, uploaded 7 March 2012]   |  

Abstract

Current methods for screening cell-material interactions typically utilize a two-dimensional (2D) culture format where cells are cultured on flat surfaces. However, there is a need for combinatorial and high-throughput screening methods to systematically screen cell-biomaterial interactions in three-dimensional (3D) tissue scaffolds for tissue engineering. Previously, we developed a two-syringe pump approach for making 3D scaffold gradients for use in combinatorial screening of salt-leached scaffolds. Herein, we demonstrate that the two-syringe pump approach can also be used to create scaffold gradients using a gas-foaming approach. Macroporous foams prepared by a gas-foaming technique are commonly used for fabrication of tissue engineering scaffolds due to their high interconnectivity and good mechanical properties. Gas-foamed scaffold gradient libraries were fabricated from two biodegradable tyrosine-derived polycarbonates: poly(desaminotyrosyl-tyrosine ethyl ester carbonate) (pDTEc) and poly(desaminotyrosyl-tyrosine octyl ester carbonate) (pDTOc). The composition of the libraries was assessed with Fourier transform infrared spectroscopy (FTIR) and showed that pDTEc/pDTOc gas-foamed scaffold gradients could be repeatably fabricated. Scanning electron microscopy showed that scaffold morphology was similar between the pDTEc-rich ends and the pDTOc-rich ends of the gradient. These results introduce a method for fabricating gas-foamed polymer scaffold gradients that can be used for combinatorial screening of cell-material interactions in 3D. View Full-Text
Keywords: combinatorial screening; polymer; scaffold; tissue engineering combinatorial screening; polymer; scaffold; tissue engineering
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chatterjee, K.; Kraigsley, A.M.; Bolikal, D.; Kohn, J.; Simon, C.G., Jr. Gas-Foamed Scaffold Gradients for Combinatorial Screening in 3D. J. Funct. Biomater. 2012, 3, 173-182.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top