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• Roy, B
• Vo Duy, S
• Puy, J
• Martin, C
• Guitton, J
• Dumontet, C
• Périgaud, C
• Lefebvre-Tournier, I
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• Roy, B
• Vo Duy, S
• Puy, J
• Martin, C
• Guitton, J
• Dumontet, C
• Périgaud, C
• Lefebvre-Tournier, I
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• Roy, B
• Vo Duy, S
• Puy, J
• Martin, C
• Guitton, J
• Dumontet, C
• Périgaud, C
• Lefebvre-Tournier, I

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J. Funct. Biomater. 2012, 3(1), 131-142; doi:10.3390/jfb3010131

Article

Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples

Béatrice Roy ¹ , Sung Vo Duy ¹ , Jean-Yves Puy ¹ , Charlotte Martin ¹ , Jérome Guitton ² , Charles Dumontet ³ , Christian Périgaud ¹  and Isabelle Lefebvre-Tournier ^1,*

¹ Institut des Biomolécules Max Mousseron, Université Montpellier 2, UMR 5247 CNRS-UM1-UM2, case courrier 1705, Place Eugène Bataillon, Montpellier Cedex 05 F-34095, France ² Laboratoire de Ciblage Thérapeutique en Cancérologie, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, Pierre Bénite F-69495, France ³ Laboratoire de Cytologie Analytique, Inserm U590, Université Lyon 1, Lyon F-69008, France

* Author to whom correspondence should be addressed.

Received: 12 January 2012; in revised form: 11 February 2012 / Accepted: 11 February 2012 / Published: 20 February 2012

(This article belongs to the Special Issue Molecularly Imprinted Polymers in Biomedical Applications)

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Abstract: A molecularly imprinted polymer (MIP) was synthesized by non-covalent imprinting polymerization using irinotecan as template. Methacrylic acid and 4-vinylpyridine were selected as functional monomers. An optimized procedure coupled to LC-PDA analysis was developed for the selective solid-phase extraction of irinotecan from various organic media. A specific capacity of 0.65 µmol•g−1 for the MIP was determined. The high specificity of this MIP was demonstrated by studying the retention behaviour of two related compounds, camptothecin and SN-38. This support was applied for the extraction of irinotecan from human serum samples.

Keywords: molecularly imprinted polymer; irinotecan; human serum; LC-PDA

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