J. Funct. Biomater. 2012, 3(1), 131-142; doi:10.3390/jfb3010131
Article

Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples

1 Institut des Biomolécules Max Mousseron, Université Montpellier 2, UMR 5247 CNRS-UM1-UM2, case courrier 1705, Place Eugène Bataillon, Montpellier Cedex 05 F-34095, France 2 Laboratoire de Ciblage Thérapeutique en Cancérologie, Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, Pierre Bénite F-69495, France 3 Laboratoire de Cytologie Analytique, Inserm U590, Université Lyon 1, Lyon F-69008, France
* Author to whom correspondence should be addressed.
Received: 12 January 2012; in revised form: 11 February 2012 / Accepted: 11 February 2012 / Published: 20 February 2012
(This article belongs to the Special Issue Molecularly Imprinted Polymers in Biomedical Applications)
PDF Full-text Download PDF Full-Text [781 KB, Updated Version, uploaded 21 February 2012 14:58 CET]
The original version is still available [781 KB, uploaded 20 February 2012 08:51 CET]
Abstract: A molecularly imprinted polymer (MIP) was synthesized by non-covalent imprinting polymerization using irinotecan as template. Methacrylic acid and 4-vinylpyridine were selected as functional monomers. An optimized procedure coupled to LC-PDA analysis was developed for the selective solid-phase extraction of irinotecan from various organic media. A specific capacity of 0.65 µmol•g−1 for the MIP was determined. The high specificity of this MIP was demonstrated by studying the retention behaviour of two related compounds, camptothecin and SN-38. This support was applied for the extraction of irinotecan from human serum samples.
Keywords: molecularly imprinted polymer; irinotecan; human serum; LC-PDA

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Roy, B.; Vo Duy, S.; Puy, J.-Y.; Martin, C.; Guitton, J.; Dumontet, C.; Périgaud, C.; Lefebvre-Tournier, I. Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples. J. Funct. Biomater. 2012, 3, 131-142.

AMA Style

Roy B, Vo Duy S, Puy J-Y, Martin C, Guitton J, Dumontet C, Périgaud C, Lefebvre-Tournier I. Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples. Journal of Functional Biomaterials. 2012; 3(1):131-142.

Chicago/Turabian Style

Roy, Béatrice; Vo Duy, Sung; Puy, Jean-Yves; Martin, Charlotte; Guitton, Jérome; Dumontet, Charles; Périgaud, Christian; Lefebvre-Tournier, Isabelle. 2012. "Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples." J. Funct. Biomater. 3, no. 1: 131-142.

J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert