J. Funct. Biomater. 2012, 3(1), 143-162; doi:10.3390/jfb3010143
Article

Anabolic Actions of the Regenerative Agent Enamel Matrix Derivative (EMD) in Oral Periosteal Fibroblasts and MG 63 Osteoblasts, Modulation by Nicotine and Glutathione in a Redox Environment

1 Periodontology, King’s College London Dental Institute, Guy’s Dental Hospital, London SE1 9RT, UK 2 Periodontology, King’s College London Dental Institute, King’s College Dental Hospital, London SE5 9RW, UK
* Author to whom correspondence should be addressed.
Received: 19 October 2011; in revised form: 20 December 2011 / Accepted: 22 February 2012 / Published: 29 February 2012
(This article belongs to the Special Issue Biomaterials for Bone Substitutes)
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Abstract: Our study seeks to explore anabolic effects of a periodontal regenerative agent enamel matrix derivative (EMD). Its modulation by nicotine and the anti-oxidant glutathione (GSH) are investigated in human periosteal fibroblasts (HPF) and MG63 osteoblasts. Androgen biomarkers of oxidative stress and healing, resulting from radiolabeled androgen substrates are assayed. This in vitro model simulates a redox environment relevant to the periodontal lesion. It aims to confirm the hypothesis that EMD is an effective regenerative agent in a typically redox environment of the periodontal lesion. Monolayer cultures of MG63 osteoblasts and HPF established in culture medium are incubated with androgen substrates, and optimal concentrations of EMD, nicotine and GSH, alone and in combination. EMD significantly enhances yields of 5α-dihydrotestosterone (DHT) an effective bioactive metabolite, alone and in combination with GSH, to overcome oxidative effects of nicotine across cultures. The ‘in vitro’ findings of this study could be extrapolated to “in vivo” applications of EMD as an adjunctive regenerative therapeutic agent in an environment of chronic inflammation and oxidative stress. Increased yields of DHT implicated in matrix synthesis and direct antioxidant capacity, confirm the potential applications for enamel matrix derivative in periodontal regenerative procedures.
Keywords: enamel matrix derivative; redox status; antioxidant; regeneration

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MDPI and ACS Style

Al-Qattan, T.; Soory, M. Anabolic Actions of the Regenerative Agent Enamel Matrix Derivative (EMD) in Oral Periosteal Fibroblasts and MG 63 Osteoblasts, Modulation by Nicotine and Glutathione in a Redox Environment. J. Funct. Biomater. 2012, 3, 143-162.

AMA Style

Al-Qattan T, Soory M. Anabolic Actions of the Regenerative Agent Enamel Matrix Derivative (EMD) in Oral Periosteal Fibroblasts and MG 63 Osteoblasts, Modulation by Nicotine and Glutathione in a Redox Environment. Journal of Functional Biomaterials. 2012; 3(1):143-162.

Chicago/Turabian Style

Al-Qattan, Tareq; Soory, Mena. 2012. "Anabolic Actions of the Regenerative Agent Enamel Matrix Derivative (EMD) in Oral Periosteal Fibroblasts and MG 63 Osteoblasts, Modulation by Nicotine and Glutathione in a Redox Environment." J. Funct. Biomater. 3, no. 1: 143-162.

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