Viruses 2014, 6(5), 2138-2154; doi:10.3390/v6052138

Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China

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Received: 19 February 2014; in revised form: 27 April 2014 / Accepted: 6 May 2014 / Published: 16 May 2014
(This article belongs to the Special Issue Viruses and Bats)
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Abstract: Bats are recognized reservoirs for many emerging zoonotic viruses of public health importance. Identifying and cataloguing the viruses of bats is a logical approach to evaluate the range of potential zoonoses of bat origin. We characterized the fecal pathogen microbiome of both insectivorous and frugivorous bats, incorporating 281 individual bats comprising 20 common species, which were sampled in three locations of Yunnan province, by combining reverse transcription polymerase chain reaction (RT-PCR) assays and next-generation sequencing. Seven individual bats were paramyxovirus-positive by RT-PCR using degenerate primers, and these paramyxoviruses were mainly classified into three genera (Rubulavirus, Henipavirus and Jeilongvirus). Various additional novel pathogens were detected in the paramyxovirus-positive bats using Illumina sequencing. A total of 7066 assembled contigs (≥200 bp) were constructed, and 105 contigs matched eukaryotic viruses (of them 103 belong to 2 vertebrate virus families, 1 insect virus, and 1 mycovirus), 17 were parasites, and 4913 were homologous to prokaryotic microorganisms. Among the 103 vertebrate viral contigs, 79 displayed low identity (<70%) to known viruses including human viruses at the amino acid level, suggesting that these belong to novel and genetically divergent viruses. Overall, the most frequently identified viruses, particularly in bats from the family Hipposideridae, were retroviruses. The present study expands our understanding of the bat virome in species commonly found in Yunnan, China, and provides insight into the overall diversity of viruses that may be capable of directly or indirectly crossing over into humans.
Keywords: bat; metagenomics; hipposideridae; pathogens; viral emergence; virome; zoonoses
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yuan, L.; Li, M.; Li, L.; Monagin, C.; Chmura, A.A.; Schneider, B.S.; Epstein, J.H.; Mei, X.; Shi, Z.; Daszak, P.; Chen, J. Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China. Viruses 2014, 6, 2138-2154.

AMA Style

Yuan L, Li M, Li L, Monagin C, Chmura AA, Schneider BS, Epstein JH, Mei X, Shi Z, Daszak P, Chen J. Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China. Viruses. 2014; 6(5):2138-2154.

Chicago/Turabian Style

Yuan, Lihong; Li, Min; Li, Linmiao; Monagin, Corina; Chmura, Aleksei A.; Schneider, Bradley S.; Epstein, Jonathan H.; Mei, Xiaolin; Shi, Zhengli; Daszak, Peter; Chen, Jinping. 2014. "Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China." Viruses 6, no. 5: 2138-2154.

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