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First Molecular Evidence for Puumala Hantavirus in Poland
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute for Novel and Emerging Infectious Diseases, Südufer 10, Greifswald - Insel Riems D-17493, Germany
Institute of Environmental Sciences, Jagiellonian University, Kraków 30-387, Poland
Computational and Molecular Population Genetics (CMPG), Institute of Ecology and Evolution, University of Bern, Bern CH-3012, Switzerland
Swiss Institute of Bioinformatics, Genopode, Lausanne CH-1015, Switzerland
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 29 November 2013; in revised form: 10 January 2014 / Accepted: 14 January 2014 / Published: 23 January 2014
Abstract: Puumala virus (PUUV) causes mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS), and is responsible for the majority of hantavirus infections of humans in Fennoscandia, Central and Western Europe. Although there are relatively many PUUV sequences available from different European countries, little is known about the presence of this virus in Poland. During population studies in 2009 a total of 45 bank voles were trapped at three sites in north-eastern Poland, namely islands on Dejguny and Dobskie Lakes and in a forest near Mikołajki. S and M segment-specific RT-PCR assays detected PUUV RNA in three animals from the Mikołajki site. The obtained partial S and M segment sequences demonstrated the highest similarity to the corresponding segments of a PUUV strain from Latvia. Analysis of chest cavity fluid samples by IgG ELISA using a yeast-expressed PUUV nucleocapsid protein resulted in the detection of two seropositive samples, both being also RT-PCR positive. Interestingly, at the trapping site in Mikołajki PUUV-positive bank voles belong to the Carpathian and Eastern genetic lineages within this species. In conclusion, we herein present the first molecular evidence for PUUV in the rodent reservoir from Poland.
Keywords: Puumala virus; Poland; bank vole; Clethrionomys (Myodes) glareolus
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Ali, H.S.; Drewes, S.; Sadowska, E.T.; Mikowska, M.; Groschup, M.H.; Heckel, G.; Koteja, P.; Ulrich, R.G. First Molecular Evidence for Puumala Hantavirus in Poland. Viruses 2014, 6, 340-353.
Ali HS, Drewes S, Sadowska ET, Mikowska M, Groschup MH, Heckel G, Koteja P, Ulrich RG. First Molecular Evidence for Puumala Hantavirus in Poland. Viruses. 2014; 6(1):340-353.
Ali, Hanan S.; Drewes, Stephan; Sadowska, Edyta T.; Mikowska, Magdalena; Groschup, Martin H.; Heckel, Gerald; Koteja, Pawel; Ulrich, Rainer G. 2014. "First Molecular Evidence for Puumala Hantavirus in Poland." Viruses 6, no. 1: 340-353.