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Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology
Consiglio per la ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Patologia Vegetale, Via C. G. Bertero 22, Rome 00156, Italy
Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
Emeritus Lead Scientist.
* Author to whom correspondence should be addressed.
Received: 15 October 2013; in revised form: 17 December 2013 / Accepted: 24 December 2013 / Published: 6 January 2014
Abstract: Next-generation high throughput sequencing technologies became available at the onset of the 21st century. They provide a highly efficient, rapid, and low cost DNA sequencing platform beyond the reach of the standard and traditional DNA sequencing technologies developed in the late 1970s. They are continually improved to become faster, more efficient and cheaper. They have been used in many fields of biology since 2004. In 2009, next-generation sequencing (NGS) technologies began to be applied to several areas of plant virology including virus/viroid genome sequencing, discovery and detection, ecology and epidemiology, replication and transcription. Identification and characterization of known and unknown viruses and/or viroids in infected plants are currently among the most successful applications of these technologies. It is expected that NGS will play very significant roles in many research and non-research areas of plant virology.
Keywords: next-generation (deep) sequencing; NGS; novel virus/viroid discovery; metagenomics; virome; transcriptome; DNA sequencing; RNA sequencing (RNA-seq.)
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MDPI and ACS Style
Barba, M.; Czosnek, H.; Hadidi, A. Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology. Viruses 2014, 6, 106-136.
Barba M, Czosnek H, Hadidi A. Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology. Viruses. 2014; 6(1):106-136.
Barba, Marina; Czosnek, Henryk; Hadidi, Ahmed. 2014. "Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology." Viruses 6, no. 1: 106-136.