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Viruses 2014, 6(1), 106-136; doi:10.3390/v6010106
Review

Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology

1,* , 2
 and 3,†
Received: 15 October 2013; in revised form: 17 December 2013 / Accepted: 24 December 2013 / Published: 6 January 2014
(This article belongs to the Special Issue Plant Viruses)
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Abstract: Next-generation high throughput sequencing technologies became available at the onset of the 21st century. They provide a highly efficient, rapid, and low cost DNA sequencing platform beyond the reach of the standard and traditional DNA sequencing technologies developed in the late 1970s. They are continually improved to become faster, more efficient and cheaper. They have been used in many fields of biology since 2004. In 2009, next-generation sequencing (NGS) technologies began to be applied to several areas of plant virology including virus/viroid genome sequencing, discovery and detection, ecology and epidemiology, replication and transcription. Identification and characterization of known and unknown viruses and/or viroids in infected plants are currently among the most successful applications of these technologies. It is expected that NGS will play very significant roles in many research and non-research areas of plant virology.
Keywords: next-generation (deep) sequencing; NGS; novel virus/viroid discovery; metagenomics; virome; transcriptome; DNA sequencing; RNA sequencing (RNA-seq.) next-generation (deep) sequencing; NGS; novel virus/viroid discovery; metagenomics; virome; transcriptome; DNA sequencing; RNA sequencing (RNA-seq.)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Barba, M.; Czosnek, H.; Hadidi, A. Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology. Viruses 2014, 6, 106-136.

AMA Style

Barba M, Czosnek H, Hadidi A. Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology. Viruses. 2014; 6(1):106-136.

Chicago/Turabian Style

Barba, Marina; Czosnek, Henryk; Hadidi, Ahmed. 2014. "Historical Perspective, Development and Applications of Next-Generation Sequencing in Plant Virology." Viruses 6, no. 1: 106-136.



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