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Int. J. Mol. Sci. 2014, 15(12), 23924-23935; doi:10.3390/ijms151223924

Candida parapsilosis Biofilm Identification by Raman Spectroscopy

1
Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, v.v.i., Královopolská 147, Brno 61264, Czech Republic
2
Department of Microbiology, Faculty of Medicine and St. Anne's Faculty Hospital, Brno 65691, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 29 November 2014 / Revised: 8 December 2014 / Accepted: 17 December 2014 / Published: 22 December 2014
(This article belongs to the Special Issue Laser Application in Life Sciences)
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Abstract

Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to individual strains (two biofilm-positive and two biofilm-negative) could be made. View Full-Text
Keywords: Raman spectroscopy; Candida parapsilosis; biofilm Raman spectroscopy; Candida parapsilosis; biofilm
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Samek, O.; Mlynariková, K.; Bernatová, S.; Ježek, J.; Krzyžánek, V.; Šiler, M.; Zemánek, P.; Růžička, F.; Holá, V.; Mahelová, M. Candida parapsilosis Biofilm Identification by Raman Spectroscopy. Int. J. Mol. Sci. 2014, 15, 23924-23935.

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