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Int. J. Mol. Sci. 2015, 16(4), 6621-6630; doi:10.3390/ijms16046621

ATR-FTIR Spectroscopy for the Assessment of Biochemical Changes in Skin Due to Cutaneous Squamous Cell Carcinoma

1
Center for Lasers and Applications, Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, São Paulo SP 05508-000, Brazil
2
School of Dentistry, Universidade de São Paulo, São Paulo SP 05508-000, Brazil
3
Instituto de Ciência e Tecnologia (ICT), Universidade Federal de São Paulo, São José dos Campos SP 12.231-280, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Herbert Schneckenburger
Received: 15 December 2014 / Revised: 18 February 2015 / Accepted: 13 March 2015 / Published: 24 March 2015
(This article belongs to the Special Issue Laser Application in Life Sciences)
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Abstract

Nonmelanoma skin cancers represent 95% of cutaneous neoplasms. Among them, squamous cell carcinoma (SCC) is the more aggressive form and shows a pattern of possible metastatic profile. In this work, we used Fourier transform infrared spectroscopy (FTIR) spectroscopy to assess the biochemical changes in normal skin caused by squamous cell carcinoma induced by multi-stage chemical carcinogenesis in mice. Changes in the absorption intensities and shifts were observed in the vibrational modes associated to proteins, indicating changes in secondary conformation in the neoplastic tissue. Hierarchical cluster analysis was performed to evaluate the potential of the technique to differentiate the spectra of neoplastic and normal skin tissue, so that the accuracy obtained for this classification was 86.4%. In this sense, attenuated total reflection (ATR)-FTIR spectroscopy provides a useful tool to complement histopathological analysis in the clinical routine for the diagnosis of cutaneous squamous cell carcinoma. View Full-Text
Keywords: squamous cell carcinoma; optical diagnosis; laser spectroscopy; ATR-FTIR spectroscopy; hierarchical cluster analysis squamous cell carcinoma; optical diagnosis; laser spectroscopy; ATR-FTIR spectroscopy; hierarchical cluster analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lima, C.A.; Goulart, V.P.; Côrrea, L.; Pereira, T.M.; Zezell, D.M. ATR-FTIR Spectroscopy for the Assessment of Biochemical Changes in Skin Due to Cutaneous Squamous Cell Carcinoma. Int. J. Mol. Sci. 2015, 16, 6621-6630.

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