Next Article in Journal
Encapsulation of Gold Nanostructures and Oil-in-Water Nanocarriers in Microgels with Biomedical Potential
Previous Article in Journal
Optimisation of Ethanol-Reflux Extraction of Saponins from Steamed Panax notoginseng by Response Surface Methodology and Evaluation of Hematopoiesis Effect
Previous Article in Special Issue
β-Glucans: Multi-Functional Modulator of Wound Healing
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(5), 1207; https://doi.org/10.3390/molecules23051207

Optimization of Ultrasound-Assisted Extraction and Structural Characterization of the Polysaccharide from Pumpkin (Cucurbita moschata) Seeds

1
College of Science, Northeast Agricultural University, Harbin 150030, China
2
College of Horticulture, Northeast Agricultural University, Harbin 150030, China
*
Author to whom correspondence should be addressed.
Academic Editor: Quan-Bin Han
Received: 31 March 2018 / Revised: 26 April 2018 / Accepted: 2 May 2018 / Published: 18 May 2018
(This article belongs to the Special Issue Advances in Natural Polysaccharides Research)
View Full-Text   |   Download PDF [4086 KB, uploaded 18 May 2018]   |  

Abstract

In the present study, ultrasound-assisted extraction (UAE) of crude polysaccharides (PSP) from pumpkin seeds was optimized by response surface method (RSM). The polysaccharide yield (2.29 ± 0.14%), which agreed closely with the theoretical predicted value 2.40%, was obtained under the optimal extraction conditions: extraction time 24 min, extraction temperature 50 °C, ultrasonic power 347 W, and liquid to solid ratio 23 mL/g. After further purification by two-step column chromatography, a novel polysaccharide (PSP-1) was isolated from pumpkin seeds. PSP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.00:4.26:5.78 with molecular weight of 3728 g/mol. 1D and 2D NMR spectroscopy analysis revealed that the backbone of PSP-1 was mainly formed by β→6)-β-d-Galp-(1→, →6)-α-d-Glcp-(1→, and →3,6)-β-d-Manp-(1→ with branching at O-3 and O-6 of →3,6)-β-d-Manp-(1→. Branch linkages were composed of α-d-Glcp-(1→ and →4)-α-d-Galp-(1→. View Full-Text
Keywords: pumpkin seeds; polysaccharides; ultrasound-assisted extraction; response surface method; structural characterization pumpkin seeds; polysaccharides; ultrasound-assisted extraction; response surface method; structural characterization
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Wang, L.; Cheng, L.; Liu, F.; Li, T.; Yu, Z.; Xu, Y.; Yang, Y. Optimization of Ultrasound-Assisted Extraction and Structural Characterization of the Polysaccharide from Pumpkin (Cucurbita moschata) Seeds. Molecules 2018, 23, 1207.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top