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Molecules 2018, 23(5), 1206; https://doi.org/10.3390/molecules23051206

Optimisation of Ethanol-Reflux Extraction of Saponins from Steamed Panax notoginseng by Response Surface Methodology and Evaluation of Hematopoiesis Effect

1
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
2
Yunnan Key Laboratory of Panax notoginseng, Kunming University of Science and Technology, Kunming 650500, China
3
Laboratory of Sustainable Utilization of Panax notoginseng Resources, State Administration of Traditional Chinese Medicine, Kunming University of Science and Technology, Kunming 650500, China
*
Author to whom correspondence should be addressed.
Received: 17 April 2018 / Revised: 9 May 2018 / Accepted: 9 May 2018 / Published: 17 May 2018
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Abstract

The present study aims to optimize the ethanol-reflux extraction conditions for extracting saponins from steamed Panax notoginseng (SPN). Four variables including the extraction time (0.5–2.5 h), ethanol concentration (50–90%), water to solid ratio (W/S, 8–16), and times of extraction (1–5) were investigated by using the Box-Behnken design response surface methodology (BBD-RSM). For each response, a second-order polynomial model with high R2 values (>0.9690) was developed using multiple linear regression analysis and the optimum conditions to maximize the yield (31.96%), content (70.49 mg/g), and antioxidant activity (EC50 value of 0.0421 mg/mL) for saponins extracted from SPN were obtained with a extraction time of 1.51 h, ethanol concentration of 60%, extraction done 3 times, and a W/S of 10. The experimental values were in good consistency with the predicted ones. In addition, the extracted SPN saponins could significantly increase the levels of blood routine parameters compared with the model group (p < 0.01) and there was no significant difference in the hematopoiesis effect between the SPN group and the SPN saponins group, of which the dose was 15 times lower than the former one. It is suggested that the SPN saponins extracted by the optimized method had similar functions of “blood tonifying” at a much lower dose. View Full-Text
Keywords: steamed Panax notoginseng; saponins; extraction; optimization; antioxidant activity; response surface methodology; hematopoiesis steamed Panax notoginseng; saponins; extraction; optimization; antioxidant activity; response surface methodology; hematopoiesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Hu, Y.; Cui, X.; Zhang, Z.; Chen, L.; Zhang, Y.; Wang, C.; Yang, X.; Qu, Y.; Xiong, Y. Optimisation of Ethanol-Reflux Extraction of Saponins from Steamed Panax notoginseng by Response Surface Methodology and Evaluation of Hematopoiesis Effect. Molecules 2018, 23, 1206.

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