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Molecules 2017, 22(8), 1268; doi:10.3390/molecules22081268

Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
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Received: 24 June 2017 / Revised: 18 July 2017 / Accepted: 26 July 2017 / Published: 29 July 2017
(This article belongs to the Special Issue Current Trends in Ginseng Research)
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Abstract

Panax notoginseng has been extensively used as a traditional Chinese medicine. In the current study, molecular cloning and characterization of PnbHLH1 transcription factor were explored in Panax notoginseng. The full length of the PnbHLH1 gene obtained by splicing was 1430 bp, encoding 321 amino acids. Prokaryotic expression vector pET-28a-PnbHLH1 was constructed and transferred into the BL21 prokaryotic expression strain. An electrophoretic mobility shift assay of PnbHLH1 protein binding to E-box cis-acting elements verified that PnbHLH1 belonged to the bHLH class transcription factor which could interact with the promoter region of the E-box core sequence. The expression levels of key genes involved in the biosynthesis of triterpenoid saponins in PnbHLH1 transgenic cells were higher than those in the wild cells. Similarly, the total saponin contents were increased in the PnbHLH1 transgenic cell lines compared with the wild cell lines. Such results suggest that the PnbHLH1 transcription factor is a positive regulator in the biosynthesis of triterpenoid saponins in Panax notoginseng. View Full-Text
Keywords: Panax; transcription factors; triterpene; saponin; over-expression Panax; transcription factors; triterpene; saponin; over-expression
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MDPI and ACS Style

Zhang, X.; Ge, F.; Deng, B.; Shah, T.; Huang, Z.; Liu, D.; Chen, C. Molecular Cloning and Characterization of PnbHLH1 Transcription Factor in Panax notoginseng. Molecules 2017, 22, 1268.

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