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Molecules 2017, 22(6), 880; doi:10.3390/molecules22060880

Yeast Extract Stimulates Ginsenoside Production in Hairy Root Cultures of American Ginseng Cultivated in Shake Flasks and Nutrient Sprinkle Bioreactors

1
Pharmaceutical Biotechnology Department, Medical University of Lodz, Muszyńskiego 1, Lodz 90-151, Poland
2
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszyńskiego l, Lodz 90-151, Poland
3
Department of Sports Medicine, Medical University of Lodz, Pomorska 251, Lodz 92-213, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Anusha Chaparala
Received: 24 March 2017 / Accepted: 23 May 2017 / Published: 26 May 2017
(This article belongs to the Special Issue Current Trends in Ginseng Research)
View Full-Text   |   Download PDF [2467 KB, uploaded 26 May 2017]   |  

Abstract

One of the most effective strategies to enhance metabolite biosynthesis and accumulation in biotechnological systems is the use of elicitation processes. This study assesses the influence of different concentrations of yeast extract (YE) on ginsenoside biosynthesis in Panax quinquefolium (American ginseng) hairy roots cultivated in shake flasks and in a nutrient sprinkle bioreactor after 3 and 7 days of elicitation. The saponin content was determined using HPLC. The maximum yield (20 mg g−1 d.w.) of the sum of six examined ginsenosides (Rb1, Rb2, Rc, Rd, Re and Rg1) in hairy roots cultivated in shake flasks was achieved after application of YE at 50 mg L−1 concentration and 3 day exposure time. The ginsenoside level was 1.57 times higher than that attained in control medium. The same conditions of elicitation (3 day time of exposure and 50 mg L−1 of YE) also favourably influenced the biosynthesis of studied saponins in bioreactor cultures. The total ginsenoside content was 32.25 mg g−1 d.w. and was higher than that achieved in control medium and in shake flasks cultures. Obtained results indicated that yeast extract can be used to increase ginsenoside production in hairy root cultures of P. quinquefolium. View Full-Text
Keywords: hairy root cultures; nutrient sprinkle bioreactor; yeast extract; elicitation hairy root cultures; nutrient sprinkle bioreactor; yeast extract; elicitation
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MDPI and ACS Style

Kochan, E.; Szymczyk, P.; Kuźma, Ł.; Lipert, A.; Szymańska, G. Yeast Extract Stimulates Ginsenoside Production in Hairy Root Cultures of American Ginseng Cultivated in Shake Flasks and Nutrient Sprinkle Bioreactors. Molecules 2017, 22, 880.

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