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Molecules 2014, 19(8), 12509-12530; doi:10.3390/molecules190812509

Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica

1
Programa de Engenharia Química, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Caixa 68502, Brazil
2
Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Caixa 68502, Brazil
*
Author to whom correspondence should be addressed.
Received: 19 May 2014 / Revised: 28 July 2014 / Accepted: 1 August 2014 / Published: 18 August 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract

Core-shell polymer particles with different properties were produced through combined suspension-emulsion polymerizations and employed as supports for immobilization of lipase B from Candida antarctica. In order to evaluate how the morphology of the particles affects the immobilization parameters, empirical models were developed to describe the performance of the biocatalysts as a function of the specific area, volume of pores and average pore diameter of the supports. It was observed that the average pore sizes did not affect the enzymatic activities in the analyzed range of pore sizes. It was also observed that the increase of the specific area (and of the volume of pores) led to higher enzyme loadings, also leading to an increase in the esterification activity, as expected. However, when the specific area (and volume of pores) increased, the hydrolytic activity and the retention of hydrolytic activity of the biocatalysts decreased, indicating the existence of diffusional limitations for some hydrolytic reactions, probably because of the high reaction rates. View Full-Text
Keywords: core-shell particles; polystyrene; immobilization; lipase B from Candida antarctica core-shell particles; polystyrene; immobilization; lipase B from Candida antarctica
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Pinto, M.C.C.; Freire, D.M.G.; Pinto, J.C. Influence of the Morphology of Core-Shell Supports on the Immobilization of Lipase B from Candida antarctica. Molecules 2014, 19, 12509-12530.

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