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Molecules 2014, 19(8), 12461-12485; doi:10.3390/molecules190812461

Trends in Protein-Based Biosensor Assemblies for Drug Screening and Pharmaceutical Kinetic Studies

CICS-UBI Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, 6201-506 Covilhã, Portugal
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Received: 28 May 2014 / Revised: 2 August 2014 / Accepted: 5 August 2014 / Published: 18 August 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract

The selection of natural and chemical compounds for potential applications in new pharmaceutical formulations constitutes a time-consuming procedure in drug screening. To overcome this issue, new devices called biosensors, have already demonstrated their versatility and capacity for routine clinical diagnosis. Designed to perform analytical analysis for the detection of a particular analyte, biosensors based on the coupling of proteins to amperometric and optical devices have shown the appropriate selectivity, sensibility and accuracy. During the last years, the exponential demand for pharmacokinetic studies in the early phases of drug development, along with the need of lower molecular weight detection, have led to new biosensor structure materials with innovative immobilization strategies. The result has been the development of smaller, more reproducible biosensors with lower detection limits, and with a drastic reduction in the required sample volumes. Therefore in order to describe the main achievements in biosensor fields, the present review has the main aim of summarizing the essential strategies used to generate these specific devices, that can provide, under physiological conditions, a credible molecule profile and assess specific pharmacokinetic parameters. View Full-Text
Keywords: biosensor; pharmacokinetics; enzymes; antibodies; plasma proteins; nano-composites biosensor; pharmacokinetics; enzymes; antibodies; plasma proteins; nano-composites
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MDPI and ACS Style

Gonçalves, A.M.; Pedro, A.Q.; Santos, F.M.; Martins, L.M.; Maia, C.J.; Queiroz, J.A.; Passarinha, L.A. Trends in Protein-Based Biosensor Assemblies for Drug Screening and Pharmaceutical Kinetic Studies. Molecules 2014, 19, 12461-12485.

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