Automation in the High-throughput Selection of Random Combinatorial Libraries—Different Approaches for Select Applications

doi:10.3390/molecules15042478

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Molecules 2010, 15(4), 2478-2490; doi:10.3390/molecules15042478

Review

Automation in the High-throughput Selection of Random Combinatorial Libraries—Different Approaches for Select Applications

Jörn Glökler^1,* , Tatjana Schütze^1,2 and Zoltán Konthur¹

¹ Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195 Berlin, Germany ² Institute for Chemistry/ Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany

* Author to whom correspondence should be addressed.

Received: 10 February 2010; in revised form: 30 March 2010 / Accepted: 6 April 2010 / Published: 8 April 2010

(This article belongs to the Special Issue High-throughput Screening)

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Abstract: Automation in combination with high throughput screening methods has revolutionised molecular biology in the last two decades. Today, many combinatorial libraries as well as several systems for automation are available. Depending on scope, budget and time, a different combination of library and experimental handling might be most effective. In this review we will discuss several concepts of combinatorial libraries and provide information as what to expect from these depending on the given context.

Keywords: phage display; SELEX; automation; random combinatorial library; in vitro evolution

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MDPI and ACS Style

Glökler, J.; Schütze, T.; Konthur, Z. Automation in the High-throughput Selection of Random Combinatorial Libraries—Different Approaches for Select Applications. Molecules 2010, 15, 2478-2490.

AMA Style

Glökler J, Schütze T, Konthur Z. Automation in the High-throughput Selection of Random Combinatorial Libraries—Different Approaches for Select Applications. Molecules. 2010; 15(4):2478-2490.

Chicago/Turabian Style

Glökler, Jörn; Schütze, Tatjana; Konthur, Zoltán. 2010. "Automation in the High-throughput Selection of Random Combinatorial Libraries—Different Approaches for Select Applications." Molecules 15, no. 4: 2478-2490.

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