Extraction of Antioxidant Compounds for Pharmaceutical Analysis

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Pharmaceutical Processes".

Deadline for manuscript submissions: 30 October 2024 | Viewed by 580

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Department of Chromatographic Methods, Maria Curie-Skłodowska University, 20-031 Lublin, Poland
Interests: antioxidant properties; chromatography; sample preparation; food chemistry
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Special Issue Information

Dear Colleagues,

The extraction of antioxidant compounds plays a pivotal role in pharmaceutical analysis, enabling the identification, characterization, and quantification of these crucial bioactive substances. Antioxidants are vital for their ability to neutralize harmful free radicals and protect against oxidative damage, making them essential for developing therapeutic agents. Advances in extraction techniques specifically tailored for antioxidant compounds in pharmaceutical analysis are very important. From traditional solvent extraction methods to innovative approaches, such as solid-phase extraction and supercritical fluid extraction, researchers continuously strive to optimize extraction efficiency, selectivity, and sustainability. By improving the extraction of antioxidant compounds, this field aims to enhance the quality control, understanding, and utilization of pharmaceutical formulations, ultimately promoting better healthcare outcomes.

This Special Issue aims to showcase the latest advances in extraction techniques that are specifically designed for antioxidant compounds in pharmaceutical analysis. Whether your research focuses on traditional solvent extraction, solid-phase extraction, supercritical fluid extraction, or any other innovative approach, we encourage you to share your valuable insights and findings. Through contributions to this Special Issue, we hope to advance extraction methodologies, improving the identification, characterization, and quantification of antioxidant compounds in pharmaceutical formulations. We look forward to receiving your contributions and fostering meaningful discussions in this important area of research.

Dr. Małgorzata Olszowy-Tomczyk
Guest Editor

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Keywords

  • traditional extraction techniques
  • assisted extraction techniques
  • antioxidant compounds
  • natural products
  • phytochemistry
  • chromatographic analysis

Published Papers (1 paper)

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Research

15 pages, 753 KiB  
Article
Impact of Drying Method and Solvent Extraction on Ethiopian Verbascum sinaiticum (Qetetina) Leaves: Metabolite Profiling and Evaluation of Antioxidant Capacity
by Alemu Belay Legesse, Shimelis Admassu Emire, Debebe Worku Dadi, Minbale Gashu Tadesse, Timilehin Martins Oyinloye and Won Byong Yoon
Processes 2024, 12(5), 914; https://doi.org/10.3390/pr12050914 - 29 Apr 2024
Viewed by 421
Abstract
The aim of this study was to evaluate the effects of different drying methods on bioactive compounds and to analyze their composition in Verbascum sinaiticum (V. sinaiticum) leaf extracts using UHPLC-ESI-QTOF-MS/MS. V. sinaiticum is traditionally used as an herbal medicine, yet [...] Read more.
The aim of this study was to evaluate the effects of different drying methods on bioactive compounds and to analyze their composition in Verbascum sinaiticum (V. sinaiticum) leaf extracts using UHPLC-ESI-QTOF-MS/MS. V. sinaiticum is traditionally used as an herbal medicine, yet it has undergone limited scientific investigations regarding its secondary metabolites. V. sinaiticum leaves were dried using oven dryers at 50 °C, 60 °C, and 70 °C, as well as a freeze dryer. The leaves were then extracted using 50% and 70% aqueous ethanol and 100% aqueous solutions. The results showed that the highest contents of TPC and TFC were observed when 70% aqueous ethanol was used during freeze drying, reaching 181.73 mg GAE/g dw and 78.57 mg CE/g dw, respectively. The strongest correlations were observed between the TFC and DPPH radical scavenging activity (0.9082), followed by TPC and ABTS assays (0.8933) and TPC and DPPH (0.8272). In the FTIR analysis, freeze drying exhibited a lower intensity of the phenolic -OH functional groups, contrasting with significant denaturation observed during oven drying at 70 °C. Metabolite analysis identified 29 compounds in V. sinaiticum leaves, further confirming the presence of 14 phenolic and flavonoid compounds, including kaempferol, catechin, gallic acid, and myricetin derivatives, consistent with the experimentally observed antioxidant capacity. This study highlights the impact of drying methods on the bioactive composition of V. sinaiticum and underscores its potential as a source of antioxidants for food, nutraceutical, and pharmaceutical applications. Full article
(This article belongs to the Special Issue Extraction of Antioxidant Compounds for Pharmaceutical Analysis)
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