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Photonics
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Fluorescence Microscopy
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Fluorescence Microscopy

A special issue of Photonics (ISSN 2304-6732). This special issue belongs to the section "Biophotonics and Biomedical Optics".

Deadline for manuscript submissions: closed (30 September 2023) | Viewed by 3749

Special Issue Editors

Dr. Sitakanta Satapathy

E-Mail Website
Guest Editor
The City College Center for Discovery and Innovation, The City University of New York, 85th St. Nicolas Terrace, New York, NY 10031 USA
Interests: fluorescensce; light-matter interactions; light-emitting systems; theoretical and experimental modelling; spectroscopic and imaging techniques; thin film optics and photonics; artificially engineered optical materials; fluorescent probes; biooptics; chemosensors
Dr. Prathmesh Deshmukh

E-Mail Website
Guest Editor
1. The Graduate Center, City University of New York, 85 St Nicholas Terrace, New York, NY 10031, USA
2. Facebook Reality Labs (Meta Platforms), Willows Road, Redmond, WA 98052, USA
Interests: fluorescence spectroscopy & imaging; strong light matter interaction at the nanoscale; theoretical and experimental nano optics and photonics; metamaterials; 2D materials & heterostructures; diffractive optics and display systems

Special Issue Information

Dear Colleagues,

“Fluorescence” is the process of emission of electromagnetic radiation, usually visible light, caused by the excitation of atoms in a material. The subject has been the foundation of several contemporary multidisciplinary topics in the fields of physics, chemistry and biology. Since the advent of fluorescent lamps, fluorescent materials have become significant in a number of research areas, ranging from materials science to immunofluorescence.

The issue of “Fluorescence” will cover a wide range of applications, such as developing smart materials with improved optical properties and spectroscopic characteristics, chemical sensors, biological detectors and mineralogy. This Special Issue welcomes methodological and applied research and review papers. Topics will include, but are not limited to:

  • Photophysics of small molecules, supramolecular materials and self-assembled organic, inorganic and bioinspired nanostructures.
  • Strong coupling and light-matter interactions at the nanoscale, exciton-polaritons and quantum coherence.
  • Development and validation of smart photoresponsive chemical, biochemical and biological materials with multiscale approaches.
  • Thin-film optics and development of artificially engineered photonic structures for enhanced light-matter interactions.
  • Theoretical modeling of light-matter interactions and cavity-quantum electrodynamics (QEDs).

Dr. Sitakanta Satapathy
Dr. Prathmesh Deshmukh 
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Photonics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescence
  • light-matter interactions
  • light-emitting systems
  • theoretical and experimental modeling
  • spectroscopic and imaging techniques
  • thin-film optics and photonics
  • artificially engineered optical materials
  • fluorescent probes
  • biooptics
  • chemosensors

Published Papers (3 papers)

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Research

13 pages, 16416 KiB  
Article
PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro
by Andrey V. Belashov, Anna A. Zhikhoreva, Anna V. Salova, Tatiana N. Belyaeva, Ilia K. Litvinov, Elena S. Kornilova and Irina V. Semenova
Photonics 2023, 10(11), 1262; https://doi.org/10.3390/photonics10111262 - 15 Nov 2023
Viewed by 944
Abstract
Variations in the fluorescence lifetimes of Radachlorin photosensitizers in HeLa and A549 cells, caused by photodynamic treatment, were studied using fluorescence lifetime imaging microscopy (FLIM). An analysis of FLIM images of the cells demonstrated a substantial decrease in the mean Radachlorin fluorescence lifetime [...] Read more.
Variations in the fluorescence lifetimes of Radachlorin photosensitizers in HeLa and A549 cells, caused by photodynamic treatment, were studied using fluorescence lifetime imaging microscopy (FLIM). An analysis of FLIM images of the cells demonstrated a substantial decrease in the mean Radachlorin fluorescence lifetime and intensity as a result of UV irradiation of the photosensitized cells at different doses, with higher doses causing a more pronounced decrease in the mean fluorescence lifetime in cells. The post-treatment decrease in Radachlorin fluorescence intensity was accompanied by the appearance of an additional rapidly decaying fluorescence component and a nonlinear decrease in the weighted fluorescence lifetime obtained from double-exponential fits of time-resolved fluorescence signals. Experiments performed in the aqueous solutions of the photosensitizer revealed similar irreversible changes in the Radachlorin fluorescence lifetime and intensity. Therefore, the observed phenomena occurred most likely due to the photodegradation of the photosensitizer molecules and can be applied for dosimetry and monitoring of irradiation doses in different areas of malignant tissues in the course of photodynamic treatment. Full article
(This article belongs to the Special Issue Fluorescence Microscopy)
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13 pages, 3504 KiB  
Article
Bimetallic Eu/Tb Complexes for Ratiometric Temperature Sensing with Unusual Enhancement of Eu Luminescence with Temperature
by Anastasiia V. Kharcheva, Alena A. Bozhko, Yulia G. Sokolovskaya, Nataliya E. Borisova, Alexey V. Ivanov and Svetlana V. Patsaeva
Photonics 2023, 10(10), 1171; https://doi.org/10.3390/photonics10101171 - 20 Oct 2023
Cited by 1 | Viewed by 947
Abstract
In this paper we describe the results of the influence of temperature in the range of 280–340 K on the luminescence of bimetallic Eu/Tb complexes with N-heterocyclic ligand L based on 2,2′-bipyridyldicarboxylic acid in acetonitrile. The experiments were carried out for systems with [...] Read more.
In this paper we describe the results of the influence of temperature in the range of 280–340 K on the luminescence of bimetallic Eu/Tb complexes with N-heterocyclic ligand L based on 2,2′-bipyridyldicarboxylic acid in acetonitrile. The experiments were carried out for systems with various Eu/Tb ratios. The stability of the complexes of the ligand L with metal M (Eu or Tb) was determined using spectrophotometric titration in acetonitrile solutions. The LM complexes’ stability constants were found to be typical for these systems; however, the stability of Eu complex is slightly higher than that for Tb. Along with rising temperature, we observed a decrease in Tb emission intensity and, at the same time, an enhancement in Eu luminescence. An explanation of Eu luminescence enhancement involves the appearance of charge transfer states, bands of which can be observed in the Eu luminescence excitation spectra as difference spectra measured with two close temperatures. The unusual Eu luminescence enhancement upon heating was observed for the first time for the complex with tetradentate O,N-type heterocyclic diamide ligand L, while an inverse phenomenon was observed with the Tb luminescence. The Eu luminescence enhancement was found earlier for various carboxylate complex salts, but not for heterocyclic coordination complexes. This allows the construction of a ratiometric luminescent thermometer in the range of 280–340 K using the ratio of luminescence intensities for Eu and Tb. The stability constants for the individual Eu and Tb complexes help us to understand the equilibrium in L:Tb:Eu complex system and shed light on plausible speciation in solution. Full article
(This article belongs to the Special Issue Fluorescence Microscopy)
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20 pages, 9873 KiB  
Article
Fluorescent Microscopy of Hot Spots Induced by Laser Heating of Iron Oxide Nanoparticles
by Anastasia Ryabova, Daria Pominova, Inessa Markova, Aleksey Nikitin, Petr Ostroverkhov, Polina Lasareva, Alevtina Semkina, Ekaterina Plotnikova, Natalia Morozova, Igor Romanishkin, Kirill Linkov, Maksim Abakumov, Andrey Pankratov, Rudolf Steiner and Victor Loschenov
Photonics 2023, 10(7), 705; https://doi.org/10.3390/photonics10070705 - 21 Jun 2023
Cited by 1 | Viewed by 1275
Abstract
Determination of the iron oxide nanoparticles (IONPs) local temperature during laser heating is important in the aspect of laser phototherapy. We have carried out theoretical modeling of IONPs local electromagnetic (EM) field enhancement and heating under the laser action near individual IONPs and [...] Read more.
Determination of the iron oxide nanoparticles (IONPs) local temperature during laser heating is important in the aspect of laser phototherapy. We have carried out theoretical modeling of IONPs local electromagnetic (EM) field enhancement and heating under the laser action near individual IONPs and ensembles of IONPs with different sizes, shapes and chemical phases. For experimental determination of IONPs temperature, we used fluorescence thermometry with rhodamine B (RhB) based on its lifetime. Depending on the IONPs shape and their location in space, a significant change in the spatial distribution of the EM field near the IONPs surface is observed. The local heating of IONPs in an ensemble reaches sufficiently high values; the relative change is about 35 °C for Fe2O3 NPs. Nevertheless, all the studied IONPs water colloids showed heating by no more than 10 °C. The heating temperature of the ensemble depends on the thermal conductivity of the medium, on which the heat dissipation depends. During laser scanning of a cell culture incubated with different types of IONPs, the temperature increase, estimated from the shortening of the RhB fluorescence lifetime, reaches more than 100 °C. Such “hot spots” within lysosomes, where IONPs predominantly reside, lead to severe cellular stress and can be used for cell therapy. Full article
(This article belongs to the Special Issue Fluorescence Microscopy)
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