Tick-Borne Bacteria in Africa: From Diagnosis to Control

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Bacterial Pathogens".

Deadline for manuscript submissions: closed (31 March 2023) | Viewed by 6490

Special Issue Editors


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Guest Editor
1. Department of Basic Sciences, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Manouba 2010, Tunisia
2. Laboratory of Microbiology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Manouba 2010, Tunisia
Interests: ticks; tick-borne pathogens; diagnosis; phylogeny; control
Special Issues, Collections and Topics in MDPI journals

E-Mail Website1 Website2
Co-Guest Editor
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772-00100, Nairobi, Kenya
Interests: mosquitoes; ticks; tick-borne pathogens; vector-borne diseases; transmission-blocking

E-Mail Website1 Website2
Co-Guest Editor
1. Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia
2. Macha Research Trust, P.O. Box 630166, Choma, Zambia
Interests: vector-borne pathogens; molecular epidemiology; zoonotic diseases; infectious diseases; one health

Special Issue Information

Dear Colleagues,

In Africa, the expansion of the human population, the simultaneous movements of domestic and wild animals, and climate change have led to the growth and diversification of emerging and re-emerging vector-borne diseases.

Ticks are vectors for many pathogenic agents, including bacteria, protozoa, and viruses. Bacterial tick-borne diseases are numerous and have a substantial impact on human and animal health in Africa; however, data on the prevalence, diversity, and distribution of vectoring pathogenic bacteria remain scarce or absent in most African countries. In addition to bacterial pathogens, ticks are known to host diverse endosymbiotic bacteria, which are highly significant for several reasons. In particular, these microbes participate in many tick biological processes, including nutrition, fitness, reproduction, development, defense against environmental stress, immunity, and the transmission of tick-borne pathogens. In this context, African studies exploring tick associations with complex microbial communities will give key insights into the successful role of ticks as vectors in Africa.

This Special Issue aims to publish works on two broad aspects: the epidemiology of pathogenic and endosymbiotic tick-borne bacteria in African countries, and the characterization of the microbiome and tick–microbiome–pathogen interactions of given tick species in the African context. Future studies of this topic should further intensively create and/or confirm molecular methods for more accurate identification and characterization of pathogenic and endosymbiotic bacteria, and describe relationships between the tick microbiome (diversity and composition) with a functional understanding of molecular events at the tick–microbiota–pathogen interface.

For this Special Issue of Pathogens dedicated to Africa, we invite you to submit original research or review articles to share and spread your scientific knowledge in order to contribute to the accurate diagnosis and effective control of these vector-borne bacterial diseases in this beautiful continent that unites us all.

We look forward to your contributions.

Dr. Mourad Ben Said
Dr. Jandouwe Villinger
Dr. Edgar Simulundu
Guest Editors

Manuscript Submission Information

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Keywords

  • Africa
  • tick-borne bacteria
  • molecular epidemiology
  • phylogeny
  • microbiome

Published Papers (3 papers)

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Research

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25 pages, 3595 KiB  
Article
Diversity and Phylogeny of Cattle Ixodid Ticks and Associated Spotted Fever Group Rickettsia spp. in Tunisia
by Myriam Kratou, Hanene Belkahia, Rachid Selmi, Rihab Andolsi, Mokhtar Dhibi, Moez Mhadhbi, Lilia Messadi and Mourad Ben Said
Pathogens 2023, 12(4), 552; https://doi.org/10.3390/pathogens12040552 - 3 Apr 2023
Cited by 1 | Viewed by 1444
Abstract
Tick-borne rickettsioses are mainly caused by obligate intracellular bacteria belonging to the spotted fever group (SFG) of the Rickettsia genus. So far, the causative agents of SFG rickettsioses have not been detected in cattle ticks from Tunisia. Therefore, the aim of this study [...] Read more.
Tick-borne rickettsioses are mainly caused by obligate intracellular bacteria belonging to the spotted fever group (SFG) of the Rickettsia genus. So far, the causative agents of SFG rickettsioses have not been detected in cattle ticks from Tunisia. Therefore, the aim of this study was to investigate the diversity and phylogeny of ticks associated with cattle from northern Tunisia and their associated Rickettsia species. Adult ticks (n = 338) were collected from cattle in northern Tunisia. The obtained ticks were identified as Hyalomma excavatum (n = 129), Rhipicephalus sanguineus sensu lato (n = 111), Hyalomma marginatum (n = 84), Hyalomma scupense (n = 12) and Hyalomma rufipes (n = 2). After DNA extraction from the ticks, 83 PCR products based on the mitochondrial 16S rRNA gene were sequenced and a total of four genotypes for Rh. sanguineus s.l., two for Hy. marginatum and Hy. excavatum and only one for Hy. scupense and Hy. rufipes were recorded, with the occurrence of one, two and three novel genotypes, respectively, for Hy. marginatum, Hy. excavatum and Rh. sanguineus s.l. mitochondrial 16S rRNA partial sequences. The tick DNA was tested for the presence of Rickettsia spp. by using PCR measurements and sequencing targeting three different genes (ompB, ompA and gltA). Of the 338 analyzed ticks, 90 (26.6%), including 38 (34.2%) Rh. sanguineus s.l., 26 (20.1%) Hy. excavatum, 25 (29.8%) Hy. marginatum and one (50%) Hy. rufipes tick, were positive for Rickettsia spp. Based on 104 partial sequences of the three analyzed genes, the BLAST analysis and phylogenetic study showed the infection of Hy. excavatum, Hy. marginatum and Rh. sanguineus s.l. tick specimens with R. massiliae, R. aeschlimannii and R. sibirica subsp. mongolitimonae and one Hy. rufipes tick specimen with R. aeschlimannii. In addition, coinfection with R. massiliae and R. aeschlimannii was reported in one Hy. marginatum and one Rh. sanguineus s.l. tick specimen, while a coinfection with R. massiliae and R. sibirica subsp. mongolitimonae was recorded in one Rh. sanguineus s.l. tick specimen. In conclusion, our study reports, for the first time in Tunisia, the infection of cattle ticks belonging to Hyalomma and Rhipicephalus genera with zoonotic Rickettsia species belonging to the SFG group. Full article
(This article belongs to the Special Issue Tick-Borne Bacteria in Africa: From Diagnosis to Control)
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15 pages, 1512 KiB  
Article
Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks
by Sobhy Abdel-Shafy, Hend H. A. M. Abdullah, Mohamed K. Elbayoumy, Bassma S. M. Elsawy, Mohamed R. Hassan, Mona S. Mahmoud, Ahmed G. Hegazi and Eman H. Abdel-Rahman
Pathogens 2022, 11(10), 1194; https://doi.org/10.3390/pathogens11101194 - 16 Oct 2022
Cited by 4 | Viewed by 2133
Abstract
Piroplasmosis and anaplasmosis are serious tick-borne diseases (TBDs) that are concerning for the public and animal health. This study aimed to detect the molecular prevalence and epidemiological risk factors of Piroplasma and Anaplasma species in animal hosts and their associated ticks in Egypt. [...] Read more.
Piroplasmosis and anaplasmosis are serious tick-borne diseases (TBDs) that are concerning for the public and animal health. This study aimed to detect the molecular prevalence and epidemiological risk factors of Piroplasma and Anaplasma species in animal hosts and their associated ticks in Egypt. A total of 234 blood samples and 95 adult ticks were collected from animal hosts (112 cattle, 38 sheep, 28 goats, 26 buffaloes, 22 donkeys, and 8 horses) from six provinces of Egypt (AL-Faiyum, AL-Giza, Beni-Suef, Al-Minufia, Al-Beheira, and Matruh). Blood and tick samples were investigated by polymerase chain reaction coupled with sequencing targeting 18S and 16S RNA genes for Piroplasma and anaplasmataceae, respectively. Statistical analysis was conducted on the potential epidemiological factors. Of the 234 animals examined, 54 (23.08%) were positive for pathogens DNA distributed among the six provinces, where 10 (4.27%) were positive for Piroplasma, 44 (18.80%) for anaplasmataceae, and 5 (2.14%) were co-infected. Co-infections were observed only in cattle as Theileria annulata and Anaplasma marginale plus Babesia bigemina, A. marginale plus B. bigemina, and T. annulata plus B. bigemina. Piroplasmosis was recorded in cattle, with significant differences between their prevalence in their tick infestation factors. Animal species, age, and tick infestation were the potential risk factors for anaplasmosis. All ticks were free from piroplasms, but they revealed high prevalence rates of 72.63% (69/95) with anaplasmataceae. We identified T. annulata, B. bigemina, and A. marginale in cattle; A. platys in buffaloes; A. marginale and A. ovis in sheep; for the first time, A. ovis in goats; and Ehrlichia sp. in Rhipicephalus annulatus ticks. Our findings confirm the significant prevalence of piroplasmosis and anaplasmosis among subclinical and carrier animals in Egypt, highlighting the importance of the government developing policies to improve animal and public health security. Full article
(This article belongs to the Special Issue Tick-Borne Bacteria in Africa: From Diagnosis to Control)
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Review

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31 pages, 572 KiB  
Review
Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control
by Agatha Kolo
Pathogens 2023, 12(5), 702; https://doi.org/10.3390/pathogens12050702 - 12 May 2023
Cited by 5 | Viewed by 2050
Abstract
Anaplasma species, belonging to the family Anaplasmataceae in the order Rickettsiales, are obligate intracellular bacteria responsible for various tick-borne diseases of veterinary and human significance worldwide. With advancements in molecular techniques, seven formal species of Anaplasma and numerous unclassified species have been described. [...] Read more.
Anaplasma species, belonging to the family Anaplasmataceae in the order Rickettsiales, are obligate intracellular bacteria responsible for various tick-borne diseases of veterinary and human significance worldwide. With advancements in molecular techniques, seven formal species of Anaplasma and numerous unclassified species have been described. In Africa, several Anaplasma species and strains have been identified in different animals and tick species. This review aims to provide an overview of the current understanding of the molecular epidemiology and genetic diversity of classified and unclassified Anaplasma species detected in animals and ticks across Africa. The review also covers control measures that have been taken to prevent anaplasmosis transmission on the continent. This information is critical when developing anaplasmosis management and control programs in Africa. Full article
(This article belongs to the Special Issue Tick-Borne Bacteria in Africa: From Diagnosis to Control)
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