Special Issue "Nanobody"

A special issue of Antibodies (ISSN 2073-4468).

Deadline for manuscript submissions: 30 November 2018

Special Issue Editors

Guest Editor
Prof. Ulrich Rothbauer

Pharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Reutlingen, Germany
Website | E-Mail
Guest Editor
Dr. Patrick Chames

Aix Marseille University, CNRS, INSERM, Institute Paoli-Calmettes, CRCM, F-13009, Marseille, France
Website | E-Mail

Special Issue Information

Dear Colleagues,

Since their first description 25 years ago, single-domain antibody fragments, derived from heavy-chain-only antibodies of camelids, so-called nanobodies, have emerged as attractive alternatives to conventional antibodies for multiple applications in biomedical research. Compared to other small antibody fragments like Fab or scFv, nanobodies have numerous advantages. First, only one domain has to be cloned and expressed to generate a fully functional binding molecule. Secondly, nanobodies are highly soluble and stable, can be easily genetically or chemically modified, and produced in various cells and/or organisms. Microbial expression systems enable the production of purified nanobodies in the mg–g range per liter of culture, thereby offering an unlimited supply of consistent binding molecules.

To date nanobodies have become outstanding tools for in vitro and in vivo imaging, as well as structural and proteome analysis. As genetically encoded intrabodies, they open new possibilities for visualization or functional studies on proteins in living cells. The recently described advances in identification of antigen-specific nanobodies from synthetic gene libraries now makes nanobody-based approaches broadly available to many researches in the field.

This Special Issue is aimed to provide an up-to-date overview of the rising field of nanobodies including generation and functionalization of nanobodies as well as their application for immunoassays, proteomics, protein crystallization and in vitro and in vivo imaging.

Prof. Ulrich Rothbauer
Dr. Patrick Chames
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antibodies is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 350 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • Nanobodies
  • Nanobody Display Technologies
  • Functionalization of nanobodies
  • Unique application of nanobodies
  • Live cell imaging
  • Super resolution microscopy
  • In vivo imaging
  • Protein crystallization chaperones

Published Papers

This special issue is now open for submission, see below for planned papers.

Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

1) Title: Parallel selection of nanobodies for multiple antigens and their transformation in application-friendly reagents

Ario de Marco; Lab for Environmental and Life Sciences, University of Nova Gorica, Slovenia

2) Title: Using nanobodies to study protein function in developing organisms

Shinya Matsuda, Gustavo Aguilar, Alessandra Vigano and Markus Affolter *;

* Biozentrum, University of Basel, Switzerland

3) Title: Crossing the barrier of life: a future for single domain antibody-mediated drug transport over the Blood-brain-barrier?

Peter Durand Skottrup; Research Bioanalysis, Global Research Technologies, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Måløv, Denmark

4) Title: Nanobodies for super resolution microscopy

Helge Ewers; Prof. for Membrane Biochemistry, Freie Universität Berlin, Germany

5) Title: Analysis of camelid single-domain antibody responses against linear peptide epitopes

Kevin Henry; National Research Council Canada

6) Title: Single-domain antibodies and their formatting to combat viral infections

Dorien De Vlieger 1,2, Iebe Rossey 1,2, Marlies Ballegeer 1,2, Bert Schepens 1,2, Xavier Saelens 1,2

1 VIB Medical Biotechnology Center, VIB, 9052 Ghent, Belgium

2 Department of Biomedical Molecular Biology, Ghent University, 9052 Ghent, Belgium

7) Title: Design of targeted fluorescent tracers for image-guided surgery: potential of nanobody-based tracers

Pieterjan Debie and Sophie Hernot; Laboratory for In vivo Cellular and Molecular Imaging (ICMI - BEFY/MIMA), Vrije Universiteit Brussel, Brussels, Belgium

8) Title: Isolation and characterization of nanobodies against a zinc-transporting P-type ATPase

Elena Longhin 1, Christina Grønberg 1, Qiaoxia Hu 1, Kasper Røjkjær Andersen 2, Nick Stub Laursen 2,*, Pontus Gourdon 1,3,*;

1 Department of Biomedical Sciences, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark

2 Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10c, DK-8000 Århus C, Denmark

3 Department of Experimental Medical Science, Lund University, Sölvegatan 19, SE-221 84 Lund, Sweden

* corresponding authors

9) Title: Isolation of nanobodies specific for influenza B hemagglutinin and the use of yeast display to structurally characterise lineage specific binding

Walter Ramage 1, Tiziano Gaiotto 1, Christina Ball 1, Paul Risley 1, George Carnell 3, Nigel Temperton 3, Chung Cheung 2, Othmar Engelhardt 2, Simon E Hufton 1 ;

1 Biotherapeutics Division, 2 Division of Virology, National Institute for Biological Standards and Control, a centre of the Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, United Kingdom

3 Infectious Diseases and Allergy group School of Pharmacy, University of Kent, Kent, ME4 4TB, United Kingdom

10) Title: Selection and characterization of specific nanobodies for analytical purposes from a semisynthetic library

Udo Conrad, IPK Gatersleben, Corrensstrasse 3, D-06466 Seeland OT Gatersleben

11) Title: One fits all: Tag-specific nanobodies and their growing application in biomedical research

Bjoern Traenkle1 and Ulrich Rothbauer1,2;

1 Pharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany

2 Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany

12) Nanobody Engineering: Toward Next Gen Immunotherapies and Immunoimaging

Timothée Chanier1 and Patrick Chames1;

1Aix Marseille Univ, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France





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