Next Article in Journal
Yeast Diversity and Physicochemical Characteristics Associated with Coffee Bean Fermentation from the Brazilian Cerrado Mineiro Region
Next Article in Special Issue
Purification of Polymer-Grade Fumaric Acid from Fermented Spent Sulfite Liquor
Previous Article in Journal
Anhydrous Ammonia Pretreatment of Corn Stover and Enzymatic Hydrolysis of Glucan from Pretreated Corn Stover
Previous Article in Special Issue
Recent Progress in the Microbial Production of Pyruvic Acid
Article Menu

Export Article

Open AccessArticle
Fermentation 2017, 3(1), 10; doi:10.3390/fermentation3010010

Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate

National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Gunnar Lidén
Received: 7 December 2016 / Revised: 22 February 2017 / Accepted: 23 February 2017 / Published: 1 March 2017
(This article belongs to the Special Issue Carboxylic Acid Production)
View Full-Text   |   Download PDF [1043 KB, uploaded 1 March 2017]   |  

Abstract

Volatile fatty acids (VFAs) can be readily produced from many anaerobic microbes and subsequently utilized as precursors to renewable biofuels and biochemicals. Megasphaera elsdenii represents a promising host for production of VFAs, butyric acid (BA) and hexanoic acid (HA). However, due to the toxicity of these acids, product removal via an extractive fermentation system is required to achieve high titers and productivities. Here, we examine multiple aspects of extractive separations to produce BA and HA from glucose and lignocellulosic hydrolysate with M. elsdenii. A mixture of oleyl alcohol and 10% (v/v) trioctylamine was selected as an extraction solvent due to its insignificant inhibitory effect on the bacteria. Batch extractive fermentations were conducted in the pH range of 5.0 to 6.5 to select the best cell growth rate and extraction efficiency combination. Subsequently, fed-batch pertractive fermentations were run over 230 h, demonstrating high BA and HA concentrations in the extracted fraction (57.2 g/L from ~190 g/L glucose) and productivity (0.26 g/L/h). To our knowledge, these are the highest combined acid titers and productivity values reported for M. elsdenii and bacterial mono-cultures from sugars. Lastly, the production of BA and HA (up to 17 g/L) from lignocellulosic sugars was demonstrated. View Full-Text
Keywords: butyric acid; hexanoic acid; caproic acid; volatile fatty acids; short-chain carboxylates; extractive fermentation; oleyl alcohol; corn stover; biochemical; biofuel butyric acid; hexanoic acid; caproic acid; volatile fatty acids; short-chain carboxylates; extractive fermentation; oleyl alcohol; corn stover; biochemical; biofuel
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Nelson, R.S.; Peterson, D.J.; Karp, E.M.; Beckham, G.T.; Salvachúa, D. Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate. Fermentation 2017, 3, 10.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Fermentation EISSN 2311-5637 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top