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Fermentation 2017, 3(1), 9; doi:10.3390/fermentation3010009

Anhydrous Ammonia Pretreatment of Corn Stover and Enzymatic Hydrolysis of Glucan from Pretreated Corn Stover

Department of Agricultural and Biosystems Engineering, Iowa State University, 3327 Elings Hall, Ames, IA 50011, USA
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Author to whom correspondence should be addressed.
Academic Editors: Thaddeus Ezeji, Mohammad J. Taherzadeh and Badal C. Saha
Received: 27 October 2016 / Revised: 15 February 2017 / Accepted: 15 February 2017 / Published: 17 February 2017
(This article belongs to the Special Issue Biofuels and Biochemicals Production)
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Abstract

As a promising alternative of fossil fuel, ethanol has been widely used. In recent years, much attention has been devoted to bioethanol production from lignocellulosic biomass. In previous research, it is found that the pretreatment method named low-moisture anhydrous ammonia (LMAA) has the advantage of high conversion efficiency and less washing requirements. The purpose of this study was to explore the optimal conditions by employing the LMAA pretreatment method. Corn stover was treated under three levels of moisture content: 20, 50, 80 w.b.% (wet basis), and three levels of particle size: <0.09, 0.09–2, >2 mm; it was also ammoniated with a loading rate of 0.1g NH3/g biomass (dry matter). Ammoniated corn stover was then subjected to different pretreatment times (24, 96, 168 h) and temperatures (20, 75, 130 °C). After pretreatment, compositional analysis and enzymatic digestibility were conducted to determine the highest glucose yield. As a result, the highest glucose yield was obtained under the condition of 96 h and 75 °C with 50 w.b.% and 0.09–2 mm of corn stover. The main findings of this study could improve the efficiency of bioethanol production processing in the near future. View Full-Text
Keywords: anhydrous ammonia; corn stover; cellulosic ethanol; low-moisture anhydrous ammonia (LMAA); pretreatment anhydrous ammonia; corn stover; cellulosic ethanol; low-moisture anhydrous ammonia (LMAA); pretreatment
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yang, M.; Zhang, W.; Rosentrater, K.A. Anhydrous Ammonia Pretreatment of Corn Stover and Enzymatic Hydrolysis of Glucan from Pretreated Corn Stover. Fermentation 2017, 3, 9.

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