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Fermentation 2016, 2(4), 18; doi:10.3390/fermentation2040018

Yeast Nanobiotechnology

1
IJRG VUB-EPFL, NanoBiotechnology & NanoMedicine (NANO), Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), Vrije Universiteit Brussel, Brussels 1050, Belgium
2
IJRG VUB-EPFL, NanoBiotechnology & NanoMedicine (NANO), Laboratory of the Physics of Living Matter, Ecole Polytechnique de Lausanne, Lausanne 1015, Switzerland
3
IJRG VUB-EPFL, NanoBiotechnology & NanoMedicine (NANO), Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), Laboratory for Protein Biochemistry and Biomolecular Engineering (L-ProBE), Ghent University, Ghent 9000, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: Badal C. Saha
Received: 6 August 2016 / Revised: 3 October 2016 / Accepted: 13 October 2016 / Published: 21 October 2016
(This article belongs to the Special Issue Yeast Biotechnology)
View Full-Text   |   Download PDF [3497 KB, uploaded 21 October 2016]   |  

Abstract

Yeast nanobiotechnology is a recent field where nanotechniques are used to manipulate and analyse yeast cells and cell constituents at the nanoscale. The aim of this review is to give an overview and discuss nanobiotechnological analysis and manipulation techniques that have been particularly applied to yeast cells. These techniques have mostly been applied to the model yeasts Saccharomyces cerevisiae and Schizosaccaromyces pombe, and the pathogenic model yeast Candida albicans. Nanoscale imaging techniques, such as Atomic Force Microscopy (AFM), super-resolution fluorescence microscopy, and electron microscopy (scanning electron microscopy (SEM), transmission electron microscopy (TEM), including electron tomography) are reviewed and discussed. Other nano-analysis methods include single-molecule and single-cell force spectroscopy and the AFM-cantilever-based nanomotion analysis of living cells. Next, an overview is given on nano/microtechniques to pattern and manipulate yeast cells. Finally, direct contact cell manipulation methods, such as AFM-based single cell manipulation and micropipette manipulation of yeast cells, as well as non-contact cell manipulation techniques, such as optical, electrical, and magnetic cells manipulation methods are reviewed. View Full-Text
Keywords: yeasts; Atomic Force Microscopy (AFM); super-resolution fluorescence microscopy; electron microscopy; force spectroscopy; nanomotion analysis; yeast cell patterning; non- and direct-contact cell manipulation; optical/magnetic tweezer; nanoscale imaging yeasts; Atomic Force Microscopy (AFM); super-resolution fluorescence microscopy; electron microscopy; force spectroscopy; nanomotion analysis; yeast cell patterning; non- and direct-contact cell manipulation; optical/magnetic tweezer; nanoscale imaging
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Willaert, R.; Kasas, S.; Devreese, B.; Dietler, G. Yeast Nanobiotechnology. Fermentation 2016, 2, 18.

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