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Fermentation 2016, 2(4), 19; doi:10.3390/fermentation2040019

Cellulase Production from Bacillus subtilis SV1 and Its Application Potential for Saccharification of Ionic Liquid Pretreated Pine Needle Biomass under One Pot Consolidated Bioprocess

School of Biotechnology, University of Jammu, Bawe Wali Rakh, Jammu 180006, India
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Academic Editor: Thaddeus Ezeji
Received: 29 August 2016 / Revised: 3 November 2016 / Accepted: 8 November 2016 / Published: 23 November 2016
(This article belongs to the Special Issue Biofuels and Biochemicals Production)
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Abstract

Pretreatment is the requisite step for the bioconversion of lignocellulosics. Since most of the pretreatment strategies are cost/energy intensive and environmentally hazardous, there is a need for the development of an environment-friendly pretreatment process. An ionic liquid (IL) based pretreatment approach has recently emerged as the most appropriate one as it can be accomplished under ambient process conditions. However, IL-pretreated biomass needs extensive washing prior to enzymatic saccharification as the enzymes may be inhibited by the residual IL. This necessitated the exploration of IL-stable saccharification enzymes (cellulases). Current study aims at optimizing the bioprocess variables viz. carbon/nitrogen sources, medium pH and fermentation time, by using a Design of Experiments approach for achieving enhanced production of ionic liquid tolerant cellulase from a bacterial isolate Bacillus subtilis SV1. The cellulase production was increased by 1.41-fold as compared to that under unoptimized conditions. IL-stable cellulase was employed for saccharification of IL (1-ethyl-3-methylimidazolium methanesulfonate) pretreated pine needle biomass in a newly designed bioprocess named as “one pot consolidated bioprocess” (OPCB), and a saccharification efficiency of 65.9% was obtained. Consolidated bioprocesses, i.e., OPCB, offer numerous techno-economic advantages over conventional multistep processes, and may potentially pave the way for successful biorefining of biomass to biofuel, and other commercial products. View Full-Text
Keywords: ionic liquid stable cellulase; Bacillus subtilis SV1; response surface methodology; pine needle biomass; ionic liquid pretreatment; one pot consolidated bioprocess ionic liquid stable cellulase; Bacillus subtilis SV1; response surface methodology; pine needle biomass; ionic liquid pretreatment; one pot consolidated bioprocess
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nargotra, P.; Vaid, S.; Bajaj, B.K. Cellulase Production from Bacillus subtilis SV1 and Its Application Potential for Saccharification of Ionic Liquid Pretreated Pine Needle Biomass under One Pot Consolidated Bioprocess. Fermentation 2016, 2, 19.

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