http://www.mdpi.com/journal/chromatography Latest open access articles published in Chromatography at http://www.mdpi.com/journal/chromatography http://www.mdpi.com/2227-9075/1/1/9 In this study, the separation parameters in micellar electrokinetic chromatography (MEKC)-laser-induced fluorescence (LIF) were optimized for the separation of red and yellow historical dyes in 20 mM borate buffer with 20 mM sodium dodecyl sulfate (SDS). Separation conditions were optimized by changing pH, organic modifier (methanol and acetonitrile) concentrations and applied voltage. The mixtures of dyes used in this study included four anthraquinone dyes (alizarin, purpurin, emodin and carmine) and six flavonoid dyes (luteolin, apigenin, kaempferol, quercetin, morin and myricetin). For this work, dyes were introduced electro-kinetically (10 kV for 5 s) into a 50 cm capillary (10 µm id) and separated using a running potential of 18, 20, 22 and 25 kV. Absolute limits of detection for most of these dyes was less than 1 pg. For dyes such as alizarin, improved detection limits were achieved at pH = 9.24; however dyes such as purpurin had significantly improved detection limits at pH = 8.0. The successful extraction and identification of a number of dyes in plants and textiles samples is also described. Chromatography 2013-03-27 1 1 Article 10.3390/chromatography1010009 9 23 2227-9075 2013-03-27 doi: 10.3390/chromatography1010009 Shokoufeh Ahmadi Douglas Craig Douglas Goltz http://www.mdpi.com/2227-9075/1/1/1 Flavonoids are bioactive constituents in Oroxylum indicum seeds, an Asian traditional remedy used for the treatment of respiratory infections. In this study the first capillary electrophoretic method for their determination is presented. By using a 25 mM borax buffer at pH 9.2 containing 10 mM SDS as detergent, the determination of seven flavonoids was feasible in only 13 min. Method validation confirmed that the assay is in accordance with ICH requirements in respect to linearity, selectivity, sensitivity, accuracy and precision. Quantitative results revealed that baicalein-7-O-gentiobioside is the most abundant flavonoid in the drug (1.19 to 5.33%), followed by other baicalein derivatives (7-O-glucoside, 7-O-glucuronide). These observations were in good qualitative and quantitative agreement with LC-MS results. Chromatography 2013-02-25 1 1 Article 10.3390/chromatography1010001 1 8 2227-9075 2013-02-25 doi: 10.3390/chromatography1010001 Anja Krüger Markus Ganzera