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J. Dev. Biol. 2014, 2(4), 198-209; doi:10.3390/jdb2040198

A Novel Three-Dimensional Wound Healing Model

1
Department of Bioengineering, UCLA School of Engineering, 420 Westwood Plaza, Los Angeles, CA 90095-1600, USA
2
Department of Materials Science and Engineering, UCLA School of Engineering, 420 Westwood Plaza, Los Angeles, CA 90095-1600, USA
*
Author to whom correspondence should be addressed.
Received: 13 October 2014 / Revised: 7 November 2014 / Accepted: 10 December 2014 / Published: 19 December 2014
(This article belongs to the Special Issue Wound Healing and Tissue Regeneration)
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Abstract

Wound healing is a well-orchestrated process, with various cells and growth factors coming into the wound bed at a specific time to influence the healing. Understanding the wound healing process is essential to generating wound healing products that help with hard-to-heal acute wounds and chronic wounds. The 2D scratch assay whereby a wound is created by scratching a confluent layer of cells on a 2D substrate is well established and used extensively but it has a major limitationit lacks the complexity of the 3D wound healing environment. Established 3D wound healing models also have many limitations. In this paper, we present a novel 3D wound healing model that closely mimics the skin wound environment to study the cell migration of fibroblasts and keratinocytes. Three major components that exist in the wound environment are introduced in this new model: collagen, fibrin, and human foreskin fibroblasts. The novel 3D model consists of a defect, representing the actual wound, created by using a biopsy punch in a 3D collagen construct. The defect is then filled with collagen or with various solutions of fibrinogen and thrombin that polymerize into a 3D fibrin clot. Fibroblasts are then added on top of the collagen and their migration into the fibrin—or collagen—filled defect is followed for nine days. Our data clearly shows that fibroblasts migrate on both collagen and fibrin defects, though slightly faster on collagen defects than on fibrin defects. This paper shows the visibility of the model by introducing a defect filled with fibrin in a 3D collagen construct, thus mimicking a wound. Ongoing work examines keratinocyte migration on the defects of a 3D construct, which consists of collagen-containing fibroblasts. The model is also used to determine the effects of various growth factors, delivered in the wound defects, on fibroblasts’ and keratinocytes’ migration into the defects. Thus this novel 3D wound healing model provides a more complex wound healing assay than existing wound models. View Full-Text
Keywords: fibrin; 3D construct; cell migration fibrin; 3D construct; cell migration
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chen, Z.J.; Yang, J.P.; Wu, B.M.; Tawil, B. A Novel Three-Dimensional Wound Healing Model. J. Dev. Biol. 2014, 2, 198-209.

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J. Dev. Biol. EISSN 2221-3759 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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