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Biomolecules 2013, 3(3), 535-552; doi:10.3390/biom3030535

Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions

Food Biotechnology Laboratory, BOKU–University of Natural Resources and Life Sciences Vienna, Muthgasse 11, Vienna 1190, Austria
These authors contributed equally to this work.
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Received: 28 June 2013 / Revised: 9 August 2013 / Accepted: 11 August 2013 / Published: 16 August 2013
(This article belongs to the Special Issue Enzymes and Their Biotechnological Applications)
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Abstract

Pyranose dehydrogenase (PDH) is a flavin-dependent sugar oxidoreductase that is limited to a rather small group of litter-degrading basidiomycetes. The enzyme is unable to utilize oxygen as an electron acceptor, using substituted benzoquinones and (organo) metal ions instead. PDH displays a broad substrate specificity and intriguing variations in regioselectivity, depending on substrate, enzyme source and reaction conditions. In contrast to the related enzyme pyranose 2-oxidase (POx), PDHs from several sources are capable of oxidizing α- or β-1→4-linked di- and oligosaccharides, including lactose. PDH from A. xanthoderma is able to perform C-1 and C-2 oxidation, producing, in addition to lactobionic acid, 2-dehydrolactose, an intermediate for the production of lactulose, whereas PDH from A. campestris oxidizes lactose nearly exclusively at the C-1 position. In this work, we present the isolation of PDH-encoding genes from A. campestris (Ac) and A. xanthoderma (Ax) and a comparison of other so far isolated PDH-sequences. Secretory overexpression of both enzymes in Pichia pastoris was successful when using their native signal sequences with yields of 371 U·L−1 for AxPDH and 35 U·L−1 for AcPDH. The pure enzymes were characterized biochemically and tested for applications in carbohydrate conversion reactions of industrial relevance. View Full-Text
Keywords: pyranose dehydrogenase; heterologous expression; agaricus; lactose conversion; lactobionic acid pyranose dehydrogenase; heterologous expression; agaricus; lactose conversion; lactobionic acid
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MDPI and ACS Style

Staudigl, P.; Krondorfer, I.; Haltrich, D.; Peterbauer, C.K. Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions. Biomolecules 2013, 3, 535-552.

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