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Biomolecules 2013, 3(3), 514-534; doi:10.3390/biom3030514
Article

Lipases Immobilization for Effective Synthesis of Biodiesel Starting from Coffee Waste Oils

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Received: 28 June 2013; in revised form: 4 August 2013 / Accepted: 6 August 2013 / Published: 13 August 2013
(This article belongs to the Special Issue Enzymes and Their Biotechnological Applications)
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Abstract: Immobilized lipases were applied to the enzymatic conversion of oils from spent coffee ground into biodiesel. Two lipases were selected for the study because of their conformational behavior analysed by Molecular Dynamics (MD) simulations taking into account that immobilization conditions affect conformational behavior of the lipases and ultimately, their efficiency upon immobilization. The enzymatic synthesis of biodiesel was initially carried out on a model substrate (triolein) in order to select the most promising immobilized biocatalysts. The results indicate that oils can be converted quantitatively within hours. The role of the nature of the immobilization support emerged as a key factor affecting reaction rate, most probably because of partition and mass transfer barriers occurring with hydrophilic solid supports. Finally, oil from spent coffee ground was transformed into biodiesel with yields ranging from 55% to 72%. The synthesis is of particular interest in the perspective of developing sustainable processes for the production of bio-fuels from food wastes and renewable materials. The enzymatic synthesis of biodiesel is carried out under mild conditions, with stoichiometric amounts of substrates (oil and methanol) and the removal of free fatty acids is not required.
Keywords: lipases; immobilization; biodiesel; oil from spent coffee ground lipases; immobilization; biodiesel; oil from spent coffee ground
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ferrario, V.; Veny, H.; De Angelis, E.; Navarini, L.; Ebert, C.; Gardossi, L. Lipases Immobilization for Effective Synthesis of Biodiesel Starting from Coffee Waste Oils. Biomolecules 2013, 3, 514-534.

AMA Style

Ferrario V, Veny H, De Angelis E, Navarini L, Ebert C, Gardossi L. Lipases Immobilization for Effective Synthesis of Biodiesel Starting from Coffee Waste Oils. Biomolecules. 2013; 3(3):514-534.

Chicago/Turabian Style

Ferrario, Valerio; Veny, Harumi; De Angelis, Elisabetta; Navarini, Luciano; Ebert, Cynthia; Gardossi, Lucia. 2013. "Lipases Immobilization for Effective Synthesis of Biodiesel Starting from Coffee Waste Oils." Biomolecules 3, no. 3: 514-534.



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