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Metabolites 2014, 4(2), 184-217; doi:10.3390/metabo4020184
Review

Rationales and Approaches for Studying Metabolism in Eukaryotic Microalgae

1
, 1
, 1
, 1
 and 2,*
1 Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany 2 Molecular Biotechnology & Systems Biology, Technical University of Kaiserslautern, Paul-Ehrlich-Str. 23, D-67663 Kaiserslautern, Germany
* Author to whom correspondence should be addressed.
Received: 7 February 2014 / Revised: 23 March 2014 / Accepted: 25 March 2014 / Published: 11 April 2014
(This article belongs to the Special Issue Metabolism in Phototrophic Prokaryotes and Algae)
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Abstract

The generation of efficient production strains is essential for the use of eukaryotic microalgae for biofuel production. Systems biology approaches including metabolite profiling on promising microalgal strains, will provide a better understanding of their metabolic networks, which is crucial for metabolic engineering efforts. Chlamydomonas reinhardtii represents a suited model system for this purpose. We give an overview to genetically amenable microalgal strains with the potential for biofuel production and provide a critical review of currently used protocols for metabolite profiling on Chlamydomonas. We provide our own experimental data to underpin the validity of the conclusions drawn.
Keywords: Chlamydomonas reinhardtii; metabolic engineering; biofuels; metabolite profiling; GC-MS; systems biology Chlamydomonas reinhardtii; metabolic engineering; biofuels; metabolite profiling; GC-MS; systems biology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Veyel, D.; Erban, A.; Fehrle, I.; Kopka, J.; Schroda, M. Rationales and Approaches for Studying Metabolism in Eukaryotic Microalgae. Metabolites 2014, 4, 184-217.

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