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Metabolites 2014, 4(2), 218-231; doi:10.3390/metabo4020218

Xanthan Gum Removal for 1H-NMR Analysis of the Intracellular Metabolome of the Bacteria Xanthomonas axonopodis pv. citri 306

1
Brazilian Biosciences National Laboratory, Rua Giuseppe Máximo Scolfaro, 10.000-Polo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970, Campinas/SP, Brazil
2
Molecular Biology Laboratory, Boldrini Children Hospital, Rua Dr. Gabriel Porto, 1270 Cidade Universitária, CEP 13083-210, Campinas/SP, Brazil
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 31 October 2013 / Revised: 5 March 2014 / Accepted: 11 April 2014 / Published: 22 April 2014
(This article belongs to the Special Issue Sample Preparation for Metabolite Analysis)
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Abstract

Xanthomonas is a genus of phytopathogenic bacteria, which produces a slimy, polysaccharide matrix known as xanthan gum, which involves, protects and helps the bacteria during host colonization. Although broadly used as a stabilizer and thickener in the cosmetic and food industries, xanthan gum can be a troubling artifact in molecular investigations due to its rheological properties. In particular, a cross-reaction between reference compounds and the xanthan gum could compromise metabolic quantification by NMR spectroscopy. Aiming at an efficient gum extraction protocol, for a 1H-NMR-based metabolic profiling study of Xanthomonas, we tested four different interventions on the broadly used methanol-chloroform extraction protocol for the intracellular metabolic contents observation. Lower limits for bacterial pellet volumes for extraction were also probed, and a strategy is illustrated with an initial analysis of X. citri’s metabolism by 1H-NMR spectroscopy. View Full-Text
Keywords: Xanthomonas; xanthan gum removal; 1H-NMR; cell extraction protocol; metabolomics of bacteria; metabolomics of microorganisms Xanthomonas; xanthan gum removal; 1H-NMR; cell extraction protocol; metabolomics of bacteria; metabolomics of microorganisms
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MDPI and ACS Style

Pegos, V.R.; Canevarolo, R.R.; Sampaio, A.P.; Balan, A.; Zeri, A.C.M. Xanthan Gum Removal for 1H-NMR Analysis of the Intracellular Metabolome of the Bacteria Xanthomonas axonopodis pv. citri 306. Metabolites 2014, 4, 218-231.

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