Next Article in Journal
Rationales and Approaches for Studying Metabolism in Eukaryotic Microalgae
Next Article in Special Issue
The Succinated Proteome of FH-Mutant Tumours
Previous Article in Journal
Application of Stable Isotope-Assisted Metabolomics for Cell Metabolism Studies
Previous Article in Special Issue
Glycerophosphoglycerol, Beta-Alanine, and Pantothenic Acid as Metabolic Companions of Glycolytic Activity and Cell Migration in Breast Cancer Cell Lines
Article Menu

Export Article

Open AccessArticle
Metabolites 2014, 4(2), 166-183; doi:10.3390/metabo4020166

Stable Isotope Tracer Analysis in Isolated Mitochondria from Mammalian Systems

Rosalind and Morris Goodman Cancer Research Centre, 1160 Pine Ave. West, Montréal H3A 1A3, Québec, Canada
Department of Biochemistry, McGill University, McIntyre Building, 3655 prom. Sir William Osler, Montréal H3G 1Y6, Québec, Canada
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 21 November 2013 / Revised: 26 February 2014 / Accepted: 24 March 2014 / Published: 10 April 2014
(This article belongs to the Special Issue Cancer Metabolomics)
View Full-Text   |   Download PDF [495 KB, uploaded 10 April 2014]   |  


Mitochondria are a focal point in metabolism, given that they play fundamental roles in catabolic, as well as anabolic reactions. Alterations in mitochondrial functions are often studied in whole cells, and metabolomics experiments using 13C-labeled substrates, coupled with mass isotopomer distribution analyses, represent a powerful approach to study global changes in cellular metabolic activities. However, little is known regarding the assessment of metabolic activities in isolated mitochondria using this technology. Studies on isolated mitochondria permit the evaluation of whether changes in cellular metabolic activities are due to modifications in the intrinsic properties of the mitochondria. Here, we present a streamlined approach to accurately determine 13C, as well as 12C enrichments in isolated mitochondria from mammalian tissues or cultured cells by GC/MS. We demonstrate the relevance of this experimental approach by assessing the effects of drugs perturbing mitochondrial functions on the mass isotopomer enrichment of metabolic intermediates. Furthermore, we investigate 13C and 12C enrichments in mitochondria isolated from cancer cells given the emerging role of metabolic alterations in supporting tumor growth. This original method will provide a very sensitive tool to perform metabolomics studies on isolated mitochondria. View Full-Text
Keywords: mitochondria; muscle; cells; stable isotope tracer analysis; citric acid cycle; rotenone; antimycin; Neu/ErbB2; cancer; GC/MS mitochondria; muscle; cells; stable isotope tracer analysis; citric acid cycle; rotenone; antimycin; Neu/ErbB2; cancer; GC/MS

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gravel, S.-P.; Andrzejewski, S.; Avizonis, D.; St-Pierre, J. Stable Isotope Tracer Analysis in Isolated Mitochondria from Mammalian Systems. Metabolites 2014, 4, 166-183.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top