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Metabolites 2014, 4(2), 232-247; doi:10.3390/metabo4020232
Article

Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation

1,†
, 2,†
, 2
 and 1,*
Received: 27 February 2014; in revised form: 14 April 2014 / Accepted: 16 April 2014 / Published: 22 April 2014
(This article belongs to the Special Issue Metabolism in Phototrophic Prokaryotes and Algae)
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Abstract: Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Non-diazotrophic strains such as the model Synechocystis sp. PCC 6803 depend on a balanced uptake and assimilation of inorganic carbon and nitrogen sources. The internal C/N ratio is sensed via the PII protein (GlnB). We analyzed metabolic changes of the DglnB mutant of Synechocystis sp. PCC 6803 under different CO2 availability. The identified metabolites provided a snapshot of the central C/N metabolism. Cells of the DglnB mutant shifted to carbon-limiting conditions, i.e. a decreased C/N ratio, showed changes in intermediates of the sugar storage and particularly of the tricarboxylic acid cycle, arginine, and glutamate metabolism. The changes of the metabolome support the notion that the PII protein is primarily regulating the N-metabolism whereas the changes in C-metabolism are probably secondary effects of the PII deletion.
Keywords: Inorganic carbon; PII-mutant; Primary metabolism; Metabolic fingerprinting; Synechocystis Inorganic carbon; PII-mutant; Primary metabolism; Metabolic fingerprinting; Synechocystis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Schwarz, D.; Orf, I.; Kopka, J.; Hagemann, M. Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation. Metabolites 2014, 4, 232-247.

AMA Style

Schwarz D, Orf I, Kopka J, Hagemann M. Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation. Metabolites. 2014; 4(2):232-247.

Chicago/Turabian Style

Schwarz, Doreen; Orf, Isabel; Kopka, Joachim; Hagemann, Martin. 2014. "Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation." Metabolites 4, no. 2: 232-247.



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