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Cosmetics 2014, 1(3), 211-221; doi:10.3390/cosmetics1030211

Evaluation of Elastin/Collagen Content in Human Dermis in-Vivo by Multiphoton Tomography—Variation with Depth and Correlation with Aging

1
Orion Concept, 100 Rue de Suède, 37100 Tours, France
2
BASF Beauty Care Solutions France SAS, 3 Rue de Seichamps, CS 71040 Pulnoy, 54272 Essey-lès-Nancy Cedex, France
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 14 March 2014 / Revised: 31 July 2014 / Accepted: 1 August 2014 / Published: 20 August 2014
(This article belongs to the Special Issue Selected Papers from ISBS/SICC 1st Joint International Congress)
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Abstract

The aim of this study was to evaluate the influence of the depth of the dermis on the measured collagen and elastin levels and to establish the correlation between the amount of these two extracellular matrix (ECM) components and age. Multiphoton Microscopy (MPM) that measures the autofluorescence (AF) and second harmonic generation (SHG) was used to quantify the levels of elastin and collagen and to determine the SAAID (SHG-to-AF Aging Index of Dermis) at two different skin depths. A 50 MHz ultrasound scanner was used for the calculation of the Sub Epidermal Non Echogenic Band (SENEB). The measurements of the skin mechanical properties were done with a cutometer. All measurements were performed on two groups of 30 healthy female volunteers. The MPM showed a decrease of the quantity of collagen and elastin as a function of depth of the dermis as well as age. The SAAID was lower for the older skin in the deeper dermis. Ultrasound imaging revealed a significant decrease of SENEB as a function of aging. The mechanical properties confirmed a loss of cutaneous elasticity and firmness. Although multiphoton microscopy is a powerful technique to study the characteristics of the dermis and its age-related damage, the location of the measurements (depth) remains very important for the validation of these variations. These variations do not seem to be homogeneous according to the part of the dermis that is studied. View Full-Text
Keywords: non-invasive imaging; multiphoton microscopy; second harmonic generation; autofluorescence; collagen; elastin; photoaging; SAAID (SHG-to-AF Aging Index of Dermis); ultrasound imaging; mechanical properties non-invasive imaging; multiphoton microscopy; second harmonic generation; autofluorescence; collagen; elastin; photoaging; SAAID (SHG-to-AF Aging Index of Dermis); ultrasound imaging; mechanical properties
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Pittet, J.-C.; Freis, O.; Vazquez-Duchêne, M.-D.; Périé, G.; Pauly, G. Evaluation of Elastin/Collagen Content in Human Dermis in-Vivo by Multiphoton Tomography—Variation with Depth and Correlation with Aging. Cosmetics 2014, 1, 211-221.

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