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Cosmetics 2014, 1(3), 140-158; doi:10.3390/cosmetics1030140
Article

Chitin-Hyaluronan Nanoparticles: A Multifunctional Carrier to Deliver Anti-Aging Active Ingredients through the Skin

1,2,3,* , 4
, 5
, 6
, 7
, 3
, 3
, 3
, 3
 and 8
1 Skin Pharmacology, Dermatology Department, 2nd University of Naples, 80138 Naples, Italy 2 Dermatology Department, China Medical University, Shenyang 110001, China 3 Research & Development, Centre of Nanoscience, Mavi Sud, s.r.l, 04011 Aprilia (Lt), Italy 4 Department of Plastic, Reconstructive and Aesthetic Surgery, CTO (Centro Traumatologico Ortopedico) Hospital, 00142 Rome, Italy 5 Institute of Macromolecular Chemistry, AS CR (Academy of Sciences of the Czech Republic), v.v.i., Prague 6, 162 06, Czech Republic 6 Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg 195251, Russia 7 Dermatological Department, University of Messina, 98100 Messina, Italy 8 Dermatological Department, University of Parma, 43100 Parma, Italy
This paper was previously presented at ISBS-SICC 1st Joint International Congress, 15–16 October 2013, Milan, Italy.
* Author to whom correspondence should be addressed.
Received: 14 May 2014 / Revised: 11 June 2014 / Accepted: 19 June 2014 / Published: 2 July 2014
(This article belongs to the Special Issue Selected Papers from ISBS/SICC 1st Joint International Congress)

Abstract

The paper describes the process to produce Chitin Nanofibril-Hyaluronan nanoparticles (CN-HA), showing their ability to easily load active ingredients, facilitate penetration through the skin layers, and increase their effectiveness and safety as an anti-aging agent. Size and characterization of CN-HA nanoparticles were determined by Scanning Electron Microscopy (SEM) and Zetasizer, while encapsulation efficiency and loading capacity of the entrapped ingredients were controlled by chromatographic and spectrophotometric methods. Safeness was evidenced on fibroblasts and keratinocytes culture viability by the MTT (Methylthiazol) assay; anti-aging activity was evaluated in vitro measuring antioxidant capacity, anti-collagenase activity, and metalloproteinase and pro-inflammatory release; efficacy was shown in vivo by a double-blind vehicle-controlled study for 60 days on 60 women affected by photo-aging. In addition, the CN-HA nanoparticles have shown interesting possibility to be used as active ingredients, for designing and making advanced medication by the electrospinning technology, as well as to produce transparent films for food packaging, by the casting method, and can be used also in their dry form as tissues or films without adding preservatives. These unusual CN-HA nanoparticles obtained from the use of raw materials of waste origin may offer an unprecedented occasion for making innovative products, ameliorating the quality of life, reducing pollution and safeguarding the environment’s integrity.
Keywords: chitin nanofibrils; skin aging emulsions; innovative beauty masks; biopolymers; skin delivery; electrospinning; casting technology; fishery by-products; biomass waste chitin nanofibrils; skin aging emulsions; innovative beauty masks; biopolymers; skin delivery; electrospinning; casting technology; fishery by-products; biomass waste
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Morganti, P.; Palombo, M.; Tishchenko, G.; Yudin, V.E.; Guarneri, F.; Cardillo, M.; Del Ciotto, P.; Carezzi, F.; Morganti, G.; Fabrizi, G. Chitin-Hyaluronan Nanoparticles: A Multifunctional Carrier to Deliver Anti-Aging Active Ingredients through the Skin. Cosmetics 2014, 1, 140-158.

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