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Nanomaterials 2017, 7(6), 124;

Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells

Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Milano I-20157, Italy
Author to whom correspondence should be addressed.
Academic Editor: Eleonore Fröhlich
Received: 28 March 2017 / Revised: 16 May 2017 / Accepted: 24 May 2017 / Published: 27 May 2017
(This article belongs to the Special Issue Cytotoxicity of Nanoparticles)
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Infections of orthopedic implants are associated with high morbidity. The emergence of antibiotic resistant strains and the tendency of microbes to form biofilms on orthopedic devices prompt the individuation of novel antimicrobial agents. Silver nanoparticles represent an interesting alternative, but their effects on bone cells need to be clarified. We focused on osteoblast-like cells and on bone marrow-mesenchymal stem cells and found that these cells are rather resistant to the cytotoxic effects of silver nanoparticles, with a half maximal inhibitory concentration around 25 µg/mL as detected by MTT assay. Within a month of treatment, osteoblast-like cells adapt to the presence of the nanoparticles by upregulating hsp70 as shown by western blot. Hsp70 overexpression correlates with the restoration of normal cell proliferation. No alterations in the extent and time requirements were detected in mesenchymal stem cell induced to differentiate in osteoblasts in the presence of silver nanoparticles. Because the concentrations of silver nanoparticles which show antimicrobial activity are lower than those exerting toxic effects on bone-forming cells in vitro, we suggest that silver nanoparticles might represent a challenging tool to fight infections in orthopedic implants. View Full-Text
Keywords: silver nanoparticles; osteoblasts; mesenchymal stem cells silver nanoparticles; osteoblasts; mesenchymal stem cells

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Castiglioni, S.; Cazzaniga, A.; Locatelli, L.; Maier, J.A.M. Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells. Nanomaterials 2017, 7, 124.

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