Next Article in Journal
Nanocellulose-Based Materials for Water Purification
Next Article in Special Issue
Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells
Previous Article in Journal
Self‐propagating Combustion Triggered Synthesis of  3D Lamellar Graphene/BaFe12O19 Composite and Its  Electromagnetic Wave Absorption Properties
Previous Article in Special Issue
Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Nanomaterials 2017, 7(3), 56;

In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide

Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Av. Joan XXIII 27-31, Barcelona 08028, Spain
Author to whom correspondence should be addressed.
Academic Editor: Eleonore Fröhlich
Received: 26 October 2016 / Revised: 25 January 2017 / Accepted: 28 February 2017 / Published: 4 March 2017
(This article belongs to the Special Issue Cytotoxicity of Nanoparticles)
View Full-Text   |   Download PDF [2070 KB, uploaded 4 March 2017]   |  


This study was designed to determine whether nano-sized ZnO has the potential to cause acute cutaneous irritation using cultured HaCaT keratinocytes and a human skin equivalent as in vitro models, compared to non-nanomaterials. Commercial nano ZnO with different sizes (50 nm and 100 nm) was characterized by dynamic light scattering (DLS) and microscopy (SEM) in different media. Nano ZnO reduced the cell viability of HaCaT in a dose-dependent and time-dependent manner, in a similar way to macro ZnO. However, the 3D-epidermis model revealed no irritation at 1 mg/mL after 24 h of exposure. In conclusion, nano-sized ZnO does not irritate skin, in a similar manner to non-nano ZnO. View Full-Text
Keywords: zinc oxide; skin irritation; cytotoxicity; keratinocytes; 3D-epidermal model zinc oxide; skin irritation; cytotoxicity; keratinocytes; 3D-epidermal model

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Vinardell, M.P.; Llanas, H.; Marics, L.; Mitjans, M. In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide. Nanomaterials 2017, 7, 56.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Nanomaterials EISSN 2079-4991 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top