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Tissue Engineering of Corneal Endothelium
Department of Ophthalmology, Tokyo Women’s Medical University Medical Center East, 2-1-10 Nishiogu, Arakawa-ku, Tokyo 116-8567, Japan
Department of Ophthalmology, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan
* Author to whom correspondence should be addressed.
Received: 27 July 2012; in revised form: 12 September 2012 / Accepted: 17 September 2012 / Published: 17 October 2012
Abstract: Human corneal endothelial cells (HCECs) do not replicate after wounding. Therefore, corneal endothelial deficiency can result in irreversible corneal edema. Descemet stripping automated endothelial keratoplasty (DSAEK) allows selective replacement of the diseased corneal endothelium. However, DSAEK requires a donor cornea and the worldwide shortage of corneas limits its application. This review presents current knowledge on the tissue engineering of corneal endothelium using cultured HCECs. We also provide our recent work on tissue engineering for DSAEK grafts using cultured HCECs. We reconstructed DSAEK grafts by seeding cultured DiI-labelled HCECs on collagen sheets. Then HCEC sheets were transplanted onto the posterior stroma after descemetorhexis in the DSAEK group. Severe stromal edema was detected in the control group, but not in the DSAEK group throughout the observation period. Fluorescein microscopy one month after surgery showed numerous DiI-labelled cells on the posterior corneal surface in the DSAEK group. Frozen sections showed a monolayer of DiI-labelled cells on Descemet’s membrane. These findings indicate that cultured adult HCECs, transplanted with DSAEK surgery, maintain corneal transparency after transplantation and suggest the feasibility of performing DSAEK with HCECs to treat endothelial dysfunction.
Keywords: review; corneal endothelium; descemet stripping automated endothelial keratoplasty; tissue engineering; transplantation
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MDPI and ACS Style
Mimura, T.; Yokoo, S.; Yamagami, S. Tissue Engineering of Corneal Endothelium. J. Funct. Biomater. 2012, 3, 726-744.
Mimura T, Yokoo S, Yamagami S. Tissue Engineering of Corneal Endothelium. Journal of Functional Biomaterials. 2012; 3(4):726-744.
Mimura, Tatsuya; Yokoo, Seiichi; Yamagami, Satoru. 2012. "Tissue Engineering of Corneal Endothelium." J. Funct. Biomater. 3, no. 4: 726-744.