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J. Funct. Biomater. 2012, 3(3), 544-555; doi:10.3390/jfb3030544

Effects of Medium and Temperature on Cellular Responses in the Superficial Zone of Hypo-Osmotically Challenged Articular Cartilage

2,4,*  and 1
Received: 26 June 2012 / Revised: 19 July 2012 / Accepted: 27 July 2012 / Published: 9 August 2012
(This article belongs to the Special Issue Mechanics of Cells in Context with Biomaterials)
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Osmotic loading of articular cartilage has been used to study cell-tissue interactions and mechanisms in chondrocyte volume regulation in situ. Since cell volume changes are likely to affect cell’s mechanotransduction, it is important to understand how environmental factors, such as composition of the immersion medium and temperature affect cell volume changes in situ in osmotically challenged articular cartilage. In this study, chondrocytes were imaged in situ with a confocal laser scanning microscope (CLSM) through cartilage surface before and 3 min and 120 min after a hypo-osmotic challenge. Samples were measured either in phosphate buffered saline (PBS, without glucose and Ca2+) or in Dulbecco’s modified Eagle’s medium (DMEM, with glucose and Ca2+), and at 21 °C or at 37 °C. In all groups, cell volumes increased shortly after the hypotonic challenge and then recovered back to the original volumes. At both observation time points, cell volume changes as a result of the osmotic challenge were similar in PBS and DMEM in both temperatures. Our results indicate that the initial chondrocyte swelling and volume recovery as a result of the hypo-osmotic challenge of cartilage are not dependent on commonly used immersion media or temperature.
Keywords: chondrocytes; medium; temperature; confocal microscopy; osmolarity chondrocytes; medium; temperature; confocal microscopy; osmolarity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huttu, M.; Turunen, S.; Sokolinski, V.; Tiitu, V.; Lammi, M.; Korhonen, R.K. Effects of Medium and Temperature on Cellular Responses in the Superficial Zone of Hypo-Osmotically Challenged Articular Cartilage. J. Funct. Biomater. 2012, 3, 544-555.

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J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert