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J. Funct. Biomater. 2012, 3(3), 556-568; doi:10.3390/jfb3030556
Article

Platelet-Rich Plasma Favors Proliferation of Canine Adipose-Derived Mesenchymal Stem Cells in Methacrylate-Endcapped Caprolactone Porous Scaffold Niches

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Received: 29 June 2012; in revised form: 30 July 2012 / Accepted: 31 July 2012 / Published: 9 August 2012
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Abstract: Osteoarticular pathologies very often require an implementation therapy to favor regeneration processes of bone, cartilage and/or tendons. Clinical approaches performed on osteoarticular complications in dogs constitute an ideal model for human clinical translational applications. The adipose-derived mesenchymal stem cells (ASCs) have already been used to accelerate and facilitate the regenerative process. ASCs can be maintained in vitro and they can be differentiated to osteocytes or chondrocytes offering a good tool for cell replacement therapies in human and veterinary medicine. Although ACSs can be easily obtained from adipose tissue, the amplification process is usually performed by a time consuming process of successive passages. In this work, we use canine ASCs obtained by using a Bioreactor device under GMP cell culture conditions that produces a minimum of 30 million cells within 2 weeks. This method provides a rapid and aseptic method for production of sufficient stem cells with potential further use in clinical applications. We show that plasma rich in growth factors (PRGF) treatment positively contributes to viability and proliferation of canine ASCs into caprolactone 2-(methacryloyloxy) ethyl ester (CLMA) scaffolds. This biomaterial does not need additional modifications for cASCs attachment and proliferation. Here we propose a framework based on a combination of approaches that may contribute to increase the therapeutical capability of stem cells by the use of PRGF and compatible biomaterials for bone and connective tissue regeneration.
Keywords: adipose tissue; mesenchymal stem cells; PRGF; caprolactone; scaffold adipose tissue; mesenchymal stem cells; PRGF; caprolactone; scaffold
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Rodríguez-Jiménez, F.J.; Valdes-Sánchez, T.; Carrillo, J.M.; Rubio, M.; Monleon-Prades, M.; García-Cruz, D.M.; García, M.; Cugat, R.; Moreno-Manzano, V. Platelet-Rich Plasma Favors Proliferation of Canine Adipose-Derived Mesenchymal Stem Cells in Methacrylate-Endcapped Caprolactone Porous Scaffold Niches. J. Funct. Biomater. 2012, 3, 556-568.

AMA Style

Rodríguez-Jiménez FJ, Valdes-Sánchez T, Carrillo JM, Rubio M, Monleon-Prades M, García-Cruz DM, García M, Cugat R, Moreno-Manzano V. Platelet-Rich Plasma Favors Proliferation of Canine Adipose-Derived Mesenchymal Stem Cells in Methacrylate-Endcapped Caprolactone Porous Scaffold Niches. Journal of Functional Biomaterials. 2012; 3(3):556-568.

Chicago/Turabian Style

Rodríguez-Jiménez, Francisco Javier; Valdes-Sánchez, Teresa; Carrillo, José M.; Rubio, Mónica; Monleon-Prades, Manuel; García-Cruz, Dunia Mercedes; García, Montserrat; Cugat, Ramón; Moreno-Manzano, Victoria. 2012. "Platelet-Rich Plasma Favors Proliferation of Canine Adipose-Derived Mesenchymal Stem Cells in Methacrylate-Endcapped Caprolactone Porous Scaffold Niches." J. Funct. Biomater. 3, no. 3: 556-568.



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