Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
J. Funct. Biomater. 2011, 2(4), 391-413; doi:10.3390/jfb2040391
Article

Thrombin Production and Human Neutrophil Elastase Sequestration by Modified Cellulosic Dressings and Their Electrokinetic Analysis

*  and
Received: 26 September 2011; in revised form: 3 December 2011 / Accepted: 7 December 2011 / Published: 15 December 2011
(This article belongs to the Special Issue Biomaterials for Wound Healing)
View Full-Text   |   Download PDF [730 KB, updated 19 December 2011; original version uploaded 15 December 2011]   |   Browse Figures
Abstract: Wound healing is a complex series of biochemical and cellular events. Optimally, functional material design addresses the overlapping acute and inflammatory stages of wound healing based on molecular, cellular, and bio-compatibility issues. In this paper the issues addressed are uncontrolled hemostasis and inflammation which can interfere with the orderly flow of wound healing. In this regard, we review the serine proteases thrombin and elastase relative to dressing functionality that improves wound healing and examine the effects of charge in cotton/cellulosic dressing design on thrombin production and elastase sequestration (uptake by the wound dressing). Thrombin is central to the initiation and propagation of coagulation, and elastase is released from neutrophils that can function detrimentally in a stalled inflammatory phase characteristic of chronic wounds. Electrokinetic fiber surface properties of the biomaterials of this study were determined to correlate material charge and polarity with function relative to thrombin production and elastase sequestration. Human neutrophil elastase sequestration was assessed with an assay representative of chronic wound concentration with cotton gauze cross-linked with three types of polycarboxylic acids and one phosphorylation finish; thrombin production, which was assessed in a plasma-based assay via a fluorogenic peptide substrate, was determined for cotton, cotton-grafted chitosan, chitosan, rayon/polyester, and two kaolin-treated materials including a commercial hemorrhage control dressing (QuickClot Combat Gauze). A correlation in thrombin production to zeta potential was found. Two polycarboxylic acid cross linked and a phosphorylated cotton dressing gave high elastase sequestration.
Keywords: thrombin; human neutrophil elastase; cellulosic wound dressings; electrokinetic; hemostasis; inflammation thrombin; human neutrophil elastase; cellulosic wound dressings; electrokinetic; hemostasis; inflammation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Edwards, J.V.; Prevost, N. Thrombin Production and Human Neutrophil Elastase Sequestration by Modified Cellulosic Dressings and Their Electrokinetic Analysis. J. Funct. Biomater. 2011, 2, 391-413.

AMA Style

Edwards JV, Prevost N. Thrombin Production and Human Neutrophil Elastase Sequestration by Modified Cellulosic Dressings and Their Electrokinetic Analysis. Journal of Functional Biomaterials. 2011; 2(4):391-413.

Chicago/Turabian Style

Edwards, Judson Vincent; Prevost, Nicolette. 2011. "Thrombin Production and Human Neutrophil Elastase Sequestration by Modified Cellulosic Dressings and Their Electrokinetic Analysis." J. Funct. Biomater. 2, no. 4: 391-413.



J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert