J. Funct. Biomater. 2011, 2(4), 338-354; doi:10.3390/jfb2040338
Article

Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components

Received: 6 October 2011; in revised form: 23 November 2011 / Accepted: 24 November 2011 / Published: 2 December 2011
(This article belongs to the Special Issue Biomaterials for Wound Healing)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Chronic wounds typically have excessive levels of matrix metalloproteinases (MMPs) and proinflammatory cytokines that impair healing. Reducing these detrimental proteins may be key to healing chronic wounds. Proprietary protease blends were formulated specifically to degrade excessive amounts of proinflammatory factors that could prevent wound healing. Applications of protease-containing wound dressings to acute and chronic wounds have been observed clinically to resolve inflammation and appear to aid healing. The purpose of this study was to test in vitro a deliberate blend of proteases for the ability to deactivate or activate known proteins associated with inflammation or healing. Purified human target proteins were incubated with test and control solutions and samples removed at various time points. Blinded samples were tested using a novel infrared protein multiplex sandwich-ELISA-type array technique. Many proinflammatory proteins such as MMPs, cytokines and chemokines were susceptible to degradation. Many proteins such as growth factors, cytokines and TIMP1 were resistant to degradation. Not all proinflammatory proteins were deactivated. Family protein structure did not appear to affect susceptibility to degradation or deactivation. These results suggest that specific protease containing wound dressings appear to reduce multiple detrimental components which may disrupt their deleterious effects on the wound bed and microenvironment. By improving the wound microenvironment through the use of definitive proteases, these novel wound dressings may help transition wounds into the subsequent phase of healing.
Keywords: wound; healing; inflammation; matrix metalloproteinase; tumor necrosis factor alpha; growth factors; systems biology; natural materials
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MDPI and ACS Style

Parnell, L.K. Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components. J. Funct. Biomater. 2011, 2, 338-354.

AMA Style

Parnell LK. Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components. Journal of Functional Biomaterials. 2011; 2(4):338-354.

Chicago/Turabian Style

Parnell, Laura K.S. 2011. "Protein Degradation and Protection Observed in the Presence of Novel Wound Dressing Components." J. Funct. Biomater. 2, no. 4: 338-354.

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