Development of Recombinant Flagellar Antigens for Serological Detection of Salmonella enterica Serotypes Enteritidis, Hadar, Heidelberg, and Typhimurium in Poultry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Expression and Purification of Recombinant Salmonella H1 and H2 Flagellin Antigens
2.2. Development and Evaluation of S. enterica Enteritidis, Specific ELISA Using Recombinant FliCg,m Protein Fusion as the Detecting Antigen
3. Experimental Section
3.1. PCR amplification and Cloning of S. enterica Flagellin Genes, fliC and fljB from Salmonella Serotypes Enteritidis, Hadar, Heidelberg, and Typhimurium
Target/Name | Sequence1 | Expected Size (bp) | Distance from Fusion2 |
---|---|---|---|
fliC | F: ACA AGT CAT TAA TAC AAA CAG CC | 1500 | |
R :GGA TCC AGT AAA GAG AGG ACG TTT TG | |||
fljB | F: GGC ACA AGT AAT CAA CAC | 1500 | |
R: GGA TCC TTA ACT TAA CAG AGA CAG CA | |||
3′BPT | GGA GAC CGA GAT CAA CGC TC | −24 | |
5′ fliC | GGA AGA CAG ACG CTC GAT AG | 419 | |
5′ fljB | GGT CAG CAG CGA CAG ACT GT | 445 |
Strain | Genotype or Description | Source |
---|---|---|
E. coli BL21 (DE3) | F- dcm, ompT, hsdS(rBmB), gal λ(DE3) | [54] |
E. coli JM 109 | endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ(lac-proAB), [F’, traD36, proAB, lacIq, ZM15] | [55] |
S. Typhimurium | Poultry Isolate | [56] |
S. Enteritidis | Poultry Isolate | [56] |
S. Heidelberg | Poultry Isolate | [56] |
S. Hadar | Poultry Isolate | [56] |
Plasmid | ||
PinPoint™ Xa-1 T | blaTEM, pUC based vector with a biotin purification tag for protein fusions (Promega, Madison, WI) | [57] |
pJMZ 63 | PinPoint™ Xa-1 T with in-frame biotin fusion with S. Typhimurium fliC (i) | This Study |
pJMZ 42 | PinPoint™ Xa-1 T with in-frame biotin fusion with S. Enteritidis fliC (g,m) | This Study |
pJMZ 2 | PinPoint™ Xa-1 T with in-frame biotin fusion with S. Heidelberg fliC (r) | This Study |
pJMZ 47 | PinPoint™ Xa-1 T with in-frame biotin fusion with S. Hadar fliC (z10) | This Study |
pJMZ 5 | PinPoint™ Xa-1 T with in-frame biotin fusion with S. Typhimurium fljB (1,2) | This Study |
pJMZ 18 | PinPoint™ Xa-1 T with in-frame biotin fusion with S. Hadar fljB (e,n,x) | This Study |
3.2. Expression S. enterica Flagellin, Protein Fusions in E. coli Cloning Host BL21.
3.3. Purification of Recombinant Salmonella Flagellin Proteins with Biotin Tag
3.4. Enzyme-Linked Immunosorbent Assay
4. Conclusions
Acknowledgments
References
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Minicozzi, J.; Sanchez, S.; Lee, M.D.; Holt, P.S.; Hofacre, C.L.; Maurer, J.J. Development of Recombinant Flagellar Antigens for Serological Detection of Salmonella enterica Serotypes Enteritidis, Hadar, Heidelberg, and Typhimurium in Poultry. Agriculture 2013, 3, 381-397. https://doi.org/10.3390/agriculture3030381
Minicozzi J, Sanchez S, Lee MD, Holt PS, Hofacre CL, Maurer JJ. Development of Recombinant Flagellar Antigens for Serological Detection of Salmonella enterica Serotypes Enteritidis, Hadar, Heidelberg, and Typhimurium in Poultry. Agriculture. 2013; 3(3):381-397. https://doi.org/10.3390/agriculture3030381
Chicago/Turabian StyleMinicozzi, Joseph, Susan Sanchez, Margie D. Lee, Peter S. Holt, Charles L. Hofacre, and John J. Maurer. 2013. "Development of Recombinant Flagellar Antigens for Serological Detection of Salmonella enterica Serotypes Enteritidis, Hadar, Heidelberg, and Typhimurium in Poultry" Agriculture 3, no. 3: 381-397. https://doi.org/10.3390/agriculture3030381